Quantitative analysis of γ-oryzanol content in cold pressed rice bran oil by TLC-image analysis method

作     者：Apirak Sakunpak Jirapornchai Suksaeree Chaowalit Monton Pathamaporn Pathompak Krisana Kraisintu 

作者机构：Faculty of PharmacyRangsit University.Pathum Thani12000.Thailand Sino-Thai Traditional Medicine Research Center.Herbal Medicinal Products Research and Development Center(Cooperation between Rangsit University and Harbin Institute of Technology and Heilongjiang University of Chinese Medicine)Rangsil UniversityPathum Thani.12000Thailand Faculty of Oriental Medicine.Rangsit UniversityPathum Thani.12000Thailand 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2014年第4卷第2期

页      面：119-123页

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083202[工学-粮食、油脂及植物蛋白工程] 

基　　金：Supported by the Research Institute of Rangsit University Pathum Thani Thailand(Grant No.73/55) 

主　　题：γ-Oryzanol TLC-densitometric method TLC-image analysis method Rice bran oil Quantitative analysis 

摘      要：Objective:To develop and validate an image analysis method for quantitative analysis ofγ-oryzanol in cold pressed rice bran ***:TLC-densitometric and TLC-image analysis methods were developed,validated,and used for quantitative analysis of γ-oryzanol in cold pressed rice bran *** results obtained by these two different quantification methods were compared by paired ***:Both assays provided good linearity,accuracy,reproducibility and selectivity for determination of γ-***:The TLC-densitomelric and TLC-image analysis methods providett a similar reproducibility,accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil.A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis *** both methods were found to be equal,they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.