Possible novel roles of poly(rC)-binding protein 1 in SH-SY5Y neurocytes: an analysis using a dynamic Bayesian network

作     者：Li-Rong Huo Jian-Tao Liang Jun-Hua Zou Lan-Ying Wang Qi Li Xiao-Min Wang 

作者机构：Department of Physiology Key Laboratory for Neurodegenerative Disorders of the Ministry of Education Capital Medical University Beijing 100069 China Peking University Center of Medical Genetics Beijing 100083 China Xuan Wu Hospital of Capital Medical University Beijing 100053 China Genminix Informatics Ltd. Co. Shanghai 201210 China 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2012年第28卷第3期

页      面：282-290页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 080902[工学-电路与系统] 07[理学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported in part by the National Basic Research Program of China (2011CB504100) the National Natural Science Foundation of China (81072858) the "973" project(2007CB511902) from the Ministry of Science and Technology of the People’s Republic of China 

主　　题：PCBP1 RNA interference overexpression Bayesian network 

摘      要：Objective Poly（rC）-binding protein 1 （PCBP1） belongs to the heterogeneous nuclear ribonucleoprotein family and participates in transcriptional and translational regulation. Previous work has identified transcripts targeted by both knockdown and overexpression of PCBP1 in SH-SY5Y neuroblastoma cells using a microarray or ProteomeLabTM protein fractionation 2-dimensions （PF-2D） and quadrupole time-of-flight mass spectrometer. The present study aimed to further determine whether these altered transcripts from major pathways （such as Wnt signaling, TGF-β signaling, cell cycling, and apoptosis） and two other genes, H2AFX and H2BFS （screened by PF-2D）, have spatial relationships. Methods The genes were studied by qRT-PCR, and dynamic Bayesian network analysis was used to rebuild the coordination network of these transcripts. Results PCBP1 controlled the expression or activity of the seven transcripts. Moreover, PCBP1 indirectly regulated MAP2K2, FOS, FST, TP53 and WNT7B through H2AFX or regulated these genes through SAT. In contrast, TP53 and WNT7B are regulated by other genes. Conclusion The seven transcripts and PCBP1 are closely associated in a spatial interaction network.