Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe

作     者：Jing Zhang Ji-Long Liu 

作者机构：MRC Functional Genomics UnitDepartment of PhysiologyAnatomy and GeneticsUniversity of OxfordOxford0X13PTUnited Kingdom School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2019年第46卷第9期

页      面：423-432页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 070202[理学-粒子物理与原子核物理] 0702[理学-物理学] 

基　　金：supported by the UK Medical Research Council(Grant No.MC_UU_12021/3 and MC_U137788471) University of Oxford ShanghaiTech University 

主　　题：CTP synthase Cytoophidium Schizosaccharomyces pombe Heat-shock protein Nucleoside/nucleotide metabolism Cell biology Yeast genetics Cell compartmentalization 

摘      要：The metabolic enzyme CTP synthase(CTPS) is able to compartmentalize into filaments,termed cytoophidia,in a variety of organisms including bacteria,budding yeast,fission yeast,fruit flies and mammals.A previous study in budding yeast shows that the filament-forming process of CTPS is not sensitive to temperature shift.Here we study CTPS filamentation in the fission yeast Schizosaccharomyces pombe.To our surprise,we find that both the length and the occurrence of cytoophidia in S.pombe decrease upon cold shock or heat shock.The temperature-dependent changes of cytoophidia are fast and reversible.Taking advantage of yeast genetics,we demonstrate that heat-shock proteins are required for cytoophidium assembly in S.pombe.Temperature sensitivity of cytoophidia makes S.pombe an attractive model system for future investigations of this novel membraneless organelle.