Detection protein biomarker with gold nanoparticles functionalized hollow mesoporous Prussian blue nanoparticles as electrochemical probes

作     者：Jing Luo Ting Li Minghui Yang Jing Luo;Ting Li;Minghui Yang

作者机构：Department of General SurgeryThe Second Xiang-ya HospitalCentral South UniversityChangsha 410011China Key Laboratory of Hunan Province for Water Environment and Agriculture Product SafetyCollege of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2020年第31卷第1期

页      面：202-204页

核心收录：

学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 07[理学] 070302[理学-分析化学] 0703[理学-化学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (No.21575165) 

主　　题：God nanoparticles Prussian blue Electrochemical Peroxidase CD25 

摘      要：Gold nanoparticles functionalized hollow mesoporous Prussian blue nanoparticles(Au@HMPB NPs)were synthesized and its peroxidase-like activity was explored for electrochemical probe.The Au@HMPB NPs can reduce H2O2 low detection potential of-0.1 V with high sensitivity.After physically adsorption of antibodies onto the gold nanoparticle surface,the functionalized nanoparticles were turned into immuno-probe.The soluble a-chain of interleukin-2(IL-2)receptor(sCD25)was chosen as a model protein biomarker to test the performance of the probe.sCD25 in the samples were captured and enriched by capture anti-CD25 antibody functionalized magnetic nanospheres.Detection antibody functionalized Au@HMPB can then be linked onto the nanospheres and generate electrochemical current towards H2O2 reduction.The electrochemical responses to 1 mmol/L H2O2 was increased with the increasing concentration of CD25.