GNL-Scorer: a generalized model for predicting CRISPR on-target activity by machine learning and featurization

作     者：Jun Wang Xi Xiang Lars Bolund Xiuqing Zhang Lixin Cheng Yonglun Luo Jun Wang;Xi Xiang;Lars Bolund;Xiuqing Zhang;Lixin Cheng;Yonglun Luo

作者机构：BGI Education CenterUniversity of Chinese Academy of SciencesShenzhenChina Department of Critical Care MedicineShenzhen People’s HospitalThe Second Clinical Medicine College of jinan UniversityShenzhenChina BGI-ShenzhenShenzhenChina Lars Bolund Institute of Regenerative MedicineBGI-QingdaoBGI-ShenzhenQingdaoChina Department of BiomedicineAarhus UniversityDenmark 

出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)

年 卷 期：2020年第12卷第11期

页      面：909-911页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：This work was supported by the Lundbeck Foundation(R219-2016-1375 and R173-2014-1105) the Danish Research Council for Independent Research(DFF-1337-00128 and 9041-00317B) the Sapere Aude Young Research Talent Prize(DFF-1335-00763A) the Innovation Fund Denmark(BrainStem) Aarhus University Strategic Grant(AU-iCRISPR) the Sanming Project of Medicine in Shenzhen(SZ5M201612074) BGIResearch,and Guangdong Provincial Key Laboratory of Genome Read and Write(2017B030301011) 

主　　题：Cas9 CRISPR 

摘      要：CRISPR/Cas9 is an adaptive immunity system in bacteria and most archaea(Koonin and Makarova,2009;Horvath and Barrangou,2010).The CRISPR/Cas9 gene editing system is comprised of two key components,a small guide RNA(gRNA)and a Cas9 endonuclease(Deltcheva etal.,2011;Jineketal.,2012).