Spatiotemporal expression of leukemia inhibitory factor receptor protein during neural tube development in embryos with neural tube defects

作     者：Dong An Xiao-Wei Wei He-Nan Zhang Dan Liu Wei Ma Zheng-Wei Yuan Dong An;Xiao-Wei Wei;He-Nan Zhang;Dan Liu;Wei Ma;Zheng-Wei Yuan

作者机构：Key Laboratory of Health Ministry for Congenital MalformationShengjing HospitalChina Medical UniversityShenyangLiaoning ProvinceChina Department of PediatricsThe First Hospital of China Medical UniversityShenyangLiaoning ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2020年第15卷第4期

页      面：705-711页

核心收录：

学科分类：0710[理学-生物学] 100208[医学-临床检验诊断学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81601292(to DA),No.81671469(to ZWY) the National Basic Research Program of China(973 Program),No.2013CB945402(to ZWY) the National Key Research and Development Program of China,No.2016YFC1000505(to ZWY) 

主　　题：amniotic fluid development embryogenesis leukemia inhibitory factor receptor nerve regeneration neural tube defect placenta spatiotemporal expression spina bifida aperta spinal cord serum 

摘      要：Leukemia inhibitory factor receptor(LIFR),as a neuroregulatory cytokine receptor,generally shows a neuroprotective effect in central nervous system injuries.In this study,to understand the effect of LIFR on pathogenesis of neural tube defects,we explored spatiotemporal expression of LIFR at different stages of fetal development in normal and neural tube defect embryos.Spina bifida aperta was induced with all-trans retinoic acid on embryonic day 10 in rats,and the spatiotemporal expression of LIFR was investigated in spina bifida aperta rats and healthy rats from embryonic day 11 to 17.Real time-polymerase chain reaction and western blot assay were used to examine mRNA and protein expression of LIFR in healthy control and neural tube defect embryos.Results of the animal experiment demonstrated that expression of LIFR protein and mRNA in the spinal cords of normal rat embryos increased with embryonic development.LIFR was significantly downregulated in the spinal cords of spina bifida aperta rats compared with healthy rats from embryonic days 11 to 17.Immunohistochemical staining showed that the expression of LIFR in placenta and spinal cord in spina bifida aperta rat embryos was decreased compared with that in control embryos at embryonic day 15.Results from human embryo specimens showed that LIFR mRNA expression was significantly down-regulated in spinal cords of human fetuses with neural tube defects compared with normal controls at a gestational age of 24 to 33 weeks.The results were consistent with the down-regulation of LIFR in the animal experiments.Our study revealed spatiotemporal changes in expression of LIFR during embryonic neurulation.Thus,LIFR might play a specific role in neural tube development.All animal and human experimental procedures were approved by the Medical Ethics Committee of Shengjing Hospital of China Medical University,China(approval No.2016PS106K)on February 25,2016.