Molecular identification of a pancreatic lipase-like gene involved in sex pheromone biosynthesis of Bombyx mori
Molecular identification of a pancreatic lipase-like gene involved in sex pheromone biosynthesis of Bombyx mori作者机构：College of Plant Protection Henan Agricultural University Division of Reproductive Medicine The Second Affiliated Hospital ofHenan University of Traditional Chinese Medicine Zhengzhou 450008 China
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2014年第21卷第4期
页 面：459-468页
核心收录：
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 10[医学]
基 金：supported by the Natural Science Foundation of Henan Province The Young Teacher Foundation of Henan Province
主 题：Bombyx mori lipase RNAi sex pheromone
摘 要：Cytoplasmic lipid droplet （LD） lipolysis is regulated by pheromone biosynthe- sis activating neuropeptide （PBAN） in Bombyx mori. To elucidate the molecular mechanism of cytoplasm LD lipolysis, the pancreatic lipase-like gene in B. mori pheromone glands （PGs）, designated as B. mori pancreatic lipase-like gene （BmPLLG）, was identified in this study. Spatial expression analysis revealed that BmPLLG is a ubiquitous gene present in all studied tissues, such as PGs, brain, epidermis, egg, midgut, flight muscle and fat body. Temporal expression analysis showed that the BmPLLG transcript begins to express 96 h before eclosion （-96 h）, continues to increase, peaks in newly emerged females and steadily decreases after eclosion. Translational expression analysis of BmPLLG using a prepared antiserum demonstrated that BmPLLG was expressed in an age-dependent pat- tern at different development stages in B. mori. This finding was similar to the transcript expression pattern. Further RNA interference-mediated knockdown of BmPLLG signifi- cantly inhibited bombykol production. Overall, these results demonstrated that BmPLLG is involved in PBAN-induced sex pheromone biosynthesis and release.
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