Site-directed RNA editing(SDRE):Off-target effects and their countermeasures

作     者：Shaoshuai Mao Yajing Liu Shisheng Huang Xingxu Huang Tian Chi 

作者机构：School of Life Sciences and TechnologyShanghaiTech UniversityShanghai201210China CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai200031China University of Chinese Academy of SciencesBeijing100049China Department of ImmunobiologyYale University School of MedicineNew HavenCT 06520USA 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2019年第46卷第11期

页      面：531-535页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the start-up package of the ShanghaiTech University 

主　　题：Gene editing RNA editing Cas13 Off-target Specificity 

摘      要：Site-directed RNA editing(SDRE)is invaluable to basic research and clinical applications and has emerged as a new frontier in genome *** past few years have witnessed a surge of interest in SDRE,with SDRE tools emerging at a breathtaking ***,off-target effects of SDRE remain a tough problem,which constitutes a major hurdle to their clinical *** we discuss the diverse strategies for combating off-target editing,drawing lessons from the published studies as well as our ongoing ***,SDRE is still at its infancy,with significant challenges and exciting opportunities ahead.