Screening of natural compounds with neuronal differentiation promoting effects in a cell-based model

作     者：CHEN Tao WANG Juan LIU Mei ZHANG Lu-Yong LIAO Hong 

作者机构：National Center for Drug Screening China Pharmaceutical University 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2015年第13卷第8期

页      面：602-608页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 0703[理学-化学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：supported by the China National Key Hi-Tech Innovation Project for the R&D of Novel Drugs(No.2009ZX09302) National Natural Science Foundation of China(No.81271338) the Specialized Research Fund for the Doctoral Program of Higher Education of China(No.20130096110011) 

主　　题：Drug screening model Neuronal differentiation P19 embryonic carcinoma cells Wnt signaling pathway Traditional Chinese Medicine 

摘      要：The purpose of this study was to establish a drug screening method for small molecules extracted from traditional Chinese medicines(TCM) that have neuronal differentiation promoting effects, using P19 embryonic carcinoma cell as a cell-based model. First, the constructed plasmid(p Tα1-Luc) was transfected into P19 cells to establish a screening model. Second, several TCMs were screened using the established model and all-trans-retinoic acid as a positive control. Finally, the underlying molecular mechanism was explored using immunofluorescence staining, q T-PCR, and Western blot analysis. Our results indicated that the drug screen model was established successfully and that both honokiol and hyperoside induced P19 differentiation into neurons, with the possible molecular mechanism being modulating the Wnt signaling pathway. In conclusion, the drug screening model developed in the present study provides a rapid, cell-based screening platform for identifying natural compounds with neuronal differentiation effects.