Mechanisms underlying the vascular relaxation activities of Zingiber officinale var. rubrum in thoracic aorta of spontaneously hypertensive rats

作     者：Nadiah Razali Aidiahmad Dewa Mohd Zaini Asmawi Nornisah Mohamed Nurul Maizan Manshor Nadiah Razali;Aidiahmad Dewa;Mohd Zaini Asmawi;Nornisah Mohamed;Nurul Maizan Manshor

作者机构：Department of PhysiologySchool of Pharmaceutical SciencesUniversiti Sains Malaysia11800 MindenPenangMalaysia Department of PharmacologySchool of Pharmaceutical SciencesUniversiti Sains Malaysia11800 MindenPenangMalaysia Department of ChemistrySchool of Pharmaceutical SciencesUniversiti Sains Malaysia11800 MindenPenangMalaysia 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2020年第18卷第1期

页      面：46-58页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Fundamental Research Grant Scheme,Ministry of Higher Education,Malaysia(203/PFARMASI/6711408) MyPhD(MyBrain programme),Ministry of Higher Education,Malaysia 

主　　题：Zingiber officinale var.rubrum Vasorelaxation Vasoconstriction Aortic rings Herbal medicine 

摘      要：Objective: To evaluate vasorelaxant and vasoconstriction effects of Zingiber officinale var. rubrum(ZOVR)on live rats and isolated aortic rings of spontaneously hypertensive rats(SHRs).Methods: Extracts of ZOVR were subjected to in-vivo antihypertensive screening using noninvasive blood pressures in SHRs. The most potent extract, ZOVR petroleum ether extract(ZOP) was then fractionated using n-hexane, chloroform and water. Isolated thoracic aortic rings were harvested and subjected to vascular relaxation studies of n-hexane fraction of ZOP(HFZOP) with incubation of different antagonists such as Nω-nitro-L-arginine methyl ester(L-NAME, 10 μmol/L), indomethacin(10 μmol/L), methylene blue(10 μmol/L), atropine(1 μmol/L), glibenclamide(10 μmol/L), prazosin(0.01 μmol/L), and propranolol(1 μmol/L).Results: During the screening of various ZOVR extracts, ZOP produced the most reduction in blood pressures of SHRs and so did HFZOP. HFZOP significantly decreased phenylephrine-induced contraction and enhanced acetylcholine-induced relaxation. L-NAME, indomethacin, methylene blue, atropine, and glibenclamide significantly potentiated the vasorelaxant effects of HFZOP. Propranolol and prazosin did not alter the vasorelaxant effects of HFZOP. HFZOP significantly suppressed the Ca2+-dependent contraction and influenced the ratio of the responses to phenylephrine in Ca2+-free ***: This study demonstrates that ZOP may exert an antihypertensive effect in the SHR model. Its possible vascular relaxation mechanisms involve nitric oxide and prostacyclin release, activation of cGMP-KATP channels, stimulation of muscarinic receptors, and transmembrane calcium channel or Ca2+release from intracellular stores. Possible active compounds that contribute to the vasorelaxant effects are 6-gingerol, 8-gingerol and 6-shogaol.