Preparation and Characteristic Identification of Monoclonal Antibody Against Sulfamethazine

作     者：DING Liangjun LI Jichang FU Rui ZHOU Yanjun HUO Guicheng 

作者机构：College of Veterinary Medicine Northeast Agricuhural University Harbin Heilongjiang 150030 PRC Forestry Hospital of Jinshan Tun of Yichun Yichun Heilongiiang 150026 PRC Harbin Veterinary Research Institute CAAS Harbin Heilongjiang 150001 PRC College of Food Northeast Agricultural Unversity Harbin Heilongjiang 150030 PRC 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2006年第13卷第2期

页      面：145-148页

学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0906[农学-兽医学] 

主　　题：sulfamethazine monoclonal antibody diazotizing method 

摘      要：Two artificial antigens were synthesized successfully by diazotizing method, sulfamethazine（SM2）-human serum albumin （HSA） was used for the immunogen, and SM2-ovalbumin（OVA） was used for the coating antigen. The coupled reaction was successful by confirmation of the ultraviolet scanning spectrometer, and the conjugation ratio of SM2 with HSA and OVA was 9：1 and 15：1, respectively. Using cell-fusion and limiting dilution method to reclone 5 times to get 3 hybridoma strains, which could stably secret monoclonal antibody （Mab）, named CBT, BC4 and BB12. The subtype of BC4 Mab was IgG1 and chain, the molecular weight was 162 ku, the numbers of chromosomal were about 90, the affinity constant was 6.1 × 10^12 M^-1. No cross reactivity was seen between the Mab and the other 4 sulfonamides, as well as the 2 carries proteins. The Mab antibody had excellent stability.