Influence of the extraction parameters on the cytotoxicity test results of Mg materials

作     者：Xiaoli Liu Tingfei Xi Yudong Zheng 

作者机构：School of Materials Science and Technology University of Science and Technology Beijing Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary Studies Peking University Shenzhen Key Laboratory of Human Tissue Regeneration and Repair Shenzhen Institute Peking University 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2014年第24卷第5期

页      面：507-515页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：supported by the National Basic Research Program of China (Grant No. 2012CB619102) National Natural Science Foundation of China (No. 31370956) Shenzhen Special Funds for Development of Strategic Emerging Industries (No. JCYJ20130402172114948) 

主　　题：Mg alloys Cytotoxicity test Extraction parameters Corrosion Predictability 

摘      要：Recently more and more researchers query the predictability of cytotoxicity results of biomedical Mg alloys obtained according to ISO 10993 due to significant difference between in vitro and in vivo corrosion. This study aimed to observe the influence of different extraction parameters(time, volume/surface ratio and medium composition) on cytotoxicity results and illustrate whether more predictable results could be obtained by adjusting the extraction parameters. The results showed that longer extraction time and smaller extraction volume/surface ratio improve the sensitivity of screening Mg materials by making inferior Mg materials release relatively more ions to the extract; and more predictable results could not be obtained by the way of simply adding bovine serum albumin(BSA) into the extraction medium to the same level in vivo or simply using fetal bovine serum(FBS) directly as extraction medium, since BSA and FBS accelerated the corrosion of Mg materials during extraction and they affected the cells’ health states during the test. In order to get more predictable results, in our opinions, it is necessary to establish a database of primary cells’ hazards(metal ions, p H and H2gas) tolerance and a set of in vitro corrosion test with high similarity in vivo, which is very difficult to realize now however.