Gene expression analysis of primary normal human hepatocytes infected with human hepatitis B virus

作     者：Hyun Mi Ryu Sung Gyoo Park Sung Su Yea Won Hee Jang Young-Il Yang Guhung Jung 

作者机构：School of Biological SciencesSeoul National UniversitySeoul151-742Korea School of Biological Sciences and Institute of MicrobiologySeoul National UniversitySeoul151-742Korea The Paik-Inje Memorial Institute for Biomedical ScienceInje UniversityPusanjin-guPusan 614-735Korea Department of PathologyCollege of MedicineInje UniversityPaik HospitalPusan 614-735Korea 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第31期

页      面：4986-4995页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：Supported by a grant of the Korea Health 21 R&D Project  Ministry of Health and Welfare  Republic of Korea  No. A050145 

主　　题：cDNA microarray Primary normal human hepatocytes LT-α TRAF2 NIK NF-κB 

摘      要：AIM： To find the relationship between hepatitis B virus （HBV） and hepatocytes during the initial state of infection by cDNA microarray. METHODS： Primary normal human hepatocytes （PNHHs） were isolated and infected with HBV. From the PNHHs, RNA was isolated and inverted into complement DNA （cDNA） with Cy3- or Cy5- labeled dUTP for microarray analysis. The labeled cDNA was hybridized with microarray chip, including 4224 cDNAs. From the image of the microarray, expression profiles were produced and some of them were confirmed by RT-PCR, immunoblot analysis, and NF-κB luciferase reporter assay. RESULTS： From the cDNA microarray, we obtained 98 differentially regulated genes. Of the 98 genes, 53 were up regulated and 45 down regulated. Interestingly, in the up regulated genes, we found the TNF signaling pathway-related genes： LT-α, TRAF2, and NIK. By using RT-PCR, we confirmed the up-regulation of these genes in HepG2, HuhT, and Chang liver cells, which were transfected with pHBV1.2x, a plasmid encoding all HBV messages. Moreover, these three genes participated in HBV- mediated NF-κB activation. CONCLUSION： During the initial state of HBV infection, hepatocytes facilitate the activation of NF-κB through up regulation of LT-α, TRAF2, and NIK.