Global analysis of lysine succinylation in patchouli plant leaves
作者机构：Research Center of Chinese Herbal Resource Science and EngineeringGuangzhou University of Chinese MedicineKey Laboratory of Chinese Medicinal Resource from Lingnan(Guangzhou University of Chinese Medicine)Ministry of EducationJoint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese MedicinesGuangzhouGuangdong 510006P.R.China Guangdong Institute of Traditional Chinese MedicineGuangzhouGuangdong 510520P.R.China Key Laboratory of the Ministry of Education for Coastal and Wetland EcosystemsCollege of the Environment and EcologyXiamen UniversityXiamenFujian 361005P.R.China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2019年第6卷第1期
页 面：31-43页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：support for this research was provided in part by a grant from Project 81803657 supported by the National Natural Science Foundation of China the Guangdong Education department Key Promotion Platform Construction Project,Lingnan Key Laboratory of Chinese Medicine Resources Ministry of Education(2014KTSPT016) special funds for the Construction of Traditional Chinese Medicine in Guangdong Province(No.20181075) the earmarked fund for Guangdong Education Department Innovation Strong School Project(No.E1-KFD015181K28/2017KQNCX039)
摘 要：Lysine succinylation is a novel,naturally occurring posttranslational modification(PTM)in living *** lysine succinylation identification has been performed at the proteomic level in various species;however,the study of lysine succinylation in plant species is relatively *** plant(***(Blanco)Benth.,Lamiaceae)is a globally important industrial plant and medicinal *** the present study,lysine succinylome analysis was carried out in patchouli plants to determine the potential regulatory role of lysine succinylation in patchouli growth,development,and *** global succinylation sites and proteins in patchouli plants were screened with an immunoprecipitation affinity enrichment technique and advanced mass spectrometry-based *** bioinformatic analyses,such as function classification and enrichment,subcellular location predication,metabolic pathway enrichment and protein−protein interaction networking,were conducted to characterize the functions of the identified sites and *** total,1097 succinylation sites in 493 proteins were detected in patchouli plants,among which 466 succinylation sites in 241 proteins were repeatedly identified within three independent *** functional characterization of these proteins indicated that the tricarboxylic acid(TCA)cycle,oxidative phosphorylation,photosynthesis processes,and amino acid biosynthesis may be regulated by lysine *** addition,these succinylated proteins showed a wide subcellular location distribution,although the chloroplast and cytoplasm were the top two preferred cellular *** study suggested the important role of lysine succinylation in patchouli plant physiology and biology and could serve as a useful reference for succinylation studies in other medicinal plants.
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