Steroidal constituents from Helleborus thibetanus and their cytotoxicities

作     者：LI Yu-Ze ZHANG Hua-Wei FAN Hao LIANG Xiao-Fei SONG Bei CHEN Huan HUANG Wen-Li YUE Zheng-Gang SONG Xiao-Mei LIU Jian-Li 

作者机构：Key Laboratory of Resource Biology and Biotechnology in Western ChinaMinistry of EducationCollege of Life SciencesNorthwest UniversityXi'an 710069China Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource IndustrializationSchool of PharmacyShaanxi University of Chinese MedicineXianyang 712046China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2019年第17卷第10期

页      面：778-784页

核心收录：

学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 100706[医学-药理学] 1002[医学-临床医学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Key R&D Program of Shaanxi Province(No.2019ZDLSF04-03-02) Subject Innovation Team of Shaanxi University of Chinese Medicine(No.2019-YL12) the National Natural Science Foundations of China(No.81503195) the Natural Science Foundation of Shaanxi Province(No.2016JQ8030) the Key R&D Program of Shaanxi Province(Nos.2017SF-360 and 2018SF-324) 

主　　题：Helleborus thibetanus Steroidal constituents Structure identification Cytotoxic activity 

摘      要：Thibetanosides E-H(1-4),four new steroidal constituents including three rare sulfonates(2-4),were isolated from the roots and rhizomes of Helleborus thibetanus,together with nine known steroidal compounds(5-13).Their structures were elucidated by detailed spectroscopic analysis,including 1D and 2D NMR techniques and chemical *** this study,compounds 2-13 were evaluated for their cytotoxic activities against HCT116,A549 and HepG2 tumor cell lines in *** them,compound 8(thibetanoside C)showed cytotoxicities against A549 cells(IC50 39.6±1.9μmol·L^-1)and HepG2 cells(IC50 41.5±1.1μmol·L^-1),*** 9(23S,24S)-24-[(O-β-D-fucopyranosyl)oxy]-3β,23-dihydroxy-spirosta-5,25(27)-diene-1β-yl O-(4-O-acetyl-α-L-rhamnopyranosyl)-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-α-L-arabinopyranoside)showed cytotoxicity against HCT116 cells(IC5033.6±2.1μmol·L^-1).