Effects of volatile anesthetic preconditioning on expression of NFkB-regulated genes in aged rat myocardium

作     者：Caiyun Zhong Hong Qiu Jun Chen Hong Liu 

作者机构：School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of PathologyUniversity of California Davis HealthSacramentoCA 95817USA Department of Anesthesiologythe First Affiliated Hospital ofSoochow UniversitySuzhouJiangsu 215006China Department of Anesthesiology and Pain MedicineUniversity of California Davis HealthSacramentoCA 95817USA. 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2019年第33卷第4期

页      面：264-270页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 10[医学] 100217[医学-麻醉学] 

基　　金：supported by the Department of Anesthesiology and Pain Medicine University of California Davis Health 

主　　题：aging inflammatory apoptosis anesthesia preconditioning 

摘      要：Volatile anesthetic preconditioning has been shown to be a potent way to provide myocardium protection against ischemia/reperfusion(I/R)injury;however,this cardioprotection is lost in senescent animal models and elderly patients.NFκB-regulated genes have been linked to myocardial I/R injury and anesthetic preconditioning.Here,we investigated NFκB activation related to anesthetic preconditioning in aging rat myocardium.Isolated,Langendorff perfused rat hearts from Fischer 344 male rats,24 months old,were randomly assigned to one of the three groups.The hearts of the control group were perfused with physiologic solution without any intervention.The hearts in the I/R group were subjected to 25 minutes ischemia and followed by 60 minutes reperfusion.The hearts in the treatment group were subjected to 10 minutes 2.5% sevoflurane,followed by 20 minutes washout and by 25 minutes ischemia and 60 minutes of reperfusion,respectively.Left ventricular developed pressure(LVDP)and left ventricular enddiastolic pressure(LVEDP)were measured.Western blot analysis was used to measure inhibitor of κB(IκB)and antiapoptotic genes:A1,ILP,c-IAP-2,Bcl-2,caspase 8 and caspase 9.Ischemia and reperfusion significantly decreased LVDP and increased LVEDP in aged rat hearts.Anesthetic preconditioning with sevoflurane did not change the effects I/R on LVDP and LVEDP,despite the fact that after treatment with anesthetic preconditioning,the levels of IκB,A1,ILP,caspase 8 and caspase 9 were significantly different compared to those of the control hearts.In conclusion,anesthetic preconditioning with sevoflurane does not improve myocardial systolic and diastolic functions.Our results suggest that the activation of NFκB regulated genes is different in the senescent myocardium and could account for loss of cardioprotection with aging.