Possible mechanisms of lycopene amelioration of learning and memory impairment in rats with vascular dementia
Possible mechanisms of lycopene amelioration of learning and memory impairment in rats with vascular dementia作者机构：Department of Human AnatomyHistology and EmbryologySchool of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Department of PharmacyZhejiang Pharmaceutical CollegeNingboZhejiang ProvinceChina Key Laboratory of Brain Aging and Neurodegenerative DiseasesSchool of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina
出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)
年 卷 期：2020年第15卷第2期
页 面：332-341页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 100215[医学-康复医学与理疗学] 10[医学]
基 金：financially supported by the National Innovation and Entrepreneurship Training Project of China in 2013,No.201310392009(to XZZ) the Innovation and Entrepreneurship Training Project of Fujian Province of China in 2014,No.201410392058(to XZZ)
主 题：bilateral carotid artery ligation lycopene hippocampus learning and memory malondialdehyde neuron neuron-restrictive silencer factor reactive oxygen species superoxide dismutase
摘 要：Oxidative stress is involved in the pathogenesis of vascular dementia. Studies have shown that lycopene can significantly inhibit oxidative stress;therefore, we hypothesized that lycopene can reduce the level of oxidative stress in vascular dementia. A vascular dementia model was established by permanent bilateral ligation of common carotid arteries. The dosage groups were treated with lycopene(50, 100 and 200 mg/kg) every other day for 2 months. Rats without bilateral carotid artery ligation were prepared as a sham group. To test the ability of learning and memory, the Morris water maze was used to detect the average escape latency and the change of search strategy. Hematoxylin-eosin staining was used to observe changes of hippocampal neurons. The levels of oxidative stress factors, superoxide dismutase and malondialdehyde, were measured in the hippocampus by biochemical detection. The levels of reactive oxygen species in the hippocampus were observed by dihydroethidium staining. The distribution and expression of oxidative stress related protein, neuron-restrictive silencer factor, in hippocampal neurons were detected by immunofluorescence histochemistry and western blot assays. After 2 months of drug administration,(1) in the model group, the average escape latency was longer than that of the sham group, and the proportion of straight and tend tactics was lower than that of the sham group, and the hippocampal neurons were irregularly arranged and the cytoplasm was hyperchromatic.(2) The levels of reactive oxygen species and malondialdehyde in the hippocampus of the model group rats were increased, and the activity of superoxide dismutase was decreased.(3) Lycopene(50, 100 and 200 mg/kg) intervention improved the above changes, and the lycopene 100 mg/kg group showed the most significant improvement effect.(4) Neuron-restrictive silencer factor expression in the hippocampus was lower in the sham group and the lycopene 100 mg/kg group than in the model group.(5) The above data indicate that lycopene 100 mg/kg could protect against the learning-memory ability impairment of vascular dementia rats. The protective mechanism was achieved by inhibiting oxidative stress in the hippocampus. The experiment was approved by the Animal Ethics Committee of Fujian Medical University, China(approval No. 2014-025) in June 2014.
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