Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats

作     者：Sudipta Jena Asit Ray Diptirani Rath Ambika Sahoo Subhashree Singh Noohi Nasim Durga Madhab Kar Sanghamitra Nayak 

作者机构：Centre.for BotehologySisha O Anusandhaon(Demed to be UniesiryKalinga NagorGhatiliaBaubeur-750030 OdishaIndia Depurtment of PhuamoacologySchool of Pharmoceutical SciencesSissha O"Anusondhan(Demed to be Uniersityh Kalinga NagorGhaikiaBhubonesuoar-751003.Odisha India 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2019年第12卷第9期

页      面：416-424页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：The authors are grateful to Dr. S.C. Si  Dean  Centre of Biotechnology and Dr. M.R. Nayak  President  Siksha 'O' Anusandhan (Deemed to be University) for providing facilities and encouragement throughout 

主　　题：Curcuma angustifolia Hepatoprotective activity Carbon tetrachloride Antioxidant activity HepG2 cells 

摘      要：Objective:To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia(MECA)against carbon tetrachloride(CCl4)-induced hepatic damage in vitro and in ***:DPPH,ABTS and reducing power assays were performed to estimate the antioxidant effect of *** vitro cytotoxicity of MECA against HepG2 cells was evaluated,whereas serum biochemical parameters and levels of antioxidative enzymes were measured in vivo and in ***,histopathological studies were estimated in order to investigate the hepatoprotective efficacy of ***,GC-MS analysis of the extract was performed to identify the chemical ***:MECA exhibited strong antioxidant activity and attenuated CCl4-induced decrease in the viability of HepG2 ***,MECA significantly restored the ALT,AST,ALP,TP and albumin level in comparison with the CCl4 *** pre-treatment with MECA,effects of SOD,CAT and GSH were increased as well as lipid peroxidation amount decreased on CCl4-induced hepatotoxicity in in vitro and in vivo ***,histopathological observation confirmed that MECA reduced liver injury induced by CCl4 in ***-MS analysis confirmed the presence of bioactive constituents such as α-tocopherol(12.27%),phytol(7.61%),squalene(3.71%),β-sitosterol(2.19%),eugenol(2.59%),curcumenol(1.20%),β-elemene(1.00%)and eucalyptol(0.89%).Conclusions:MECA contains antioxidant and hepatoprotective constituents such asα-tocopherol,phytol,squalene and eugenol and exerts hepatoprotective effect both in vitro and in vivo.