Optical detection of acetylcholine esterase based on CdTe quantum dots

作     者：CHEN ZhenZhen REN XiangLing TANG FangQiong 

作者机构：Laboratory of Controllable Preparation and Application of Nanomaterials Technical Institute of Physics and Chemistry Chinese Academy of Sciences 

出 版 物：《Chinese Science Bulletin》 (Chinese Science Bulletin)

年 卷 期：2013年第58卷第21期

页      面：2622-2627页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 08[工学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (81171454 and 61178035) 

主　　题：乙酰胆碱酯酶 量子点 光学检测 CdTe 生物传感器 传感器检测 TGA AChE 

摘      要：In this study, we have constructed a simple, sensitive and rapid biosensor for detection of acetylcholine esterase (AChE) based on CdTe quantum dots (QDs). The detection limit of AChE by one-step enzyme reaction based on the thiolglycolic acid (TGA) stabilized QDs (TGA-QDs) was 10 U/L and the linear range was 10-100 and 100-1200 U/L, respectively. The detection limit of AChE by two-step enzyme reaction based on the 3-mercaptopropionic acid (MGA) stabilized QDs (MGA-QDs) was found to be 20 U/L and the linear range was 100-2500 U/L. The experimental conditions of biosensors were optimized, and the detection mechanism was studied. We also detected AChE in serum samples based on TGA-QDs or MGA-QDs. The linear range was 10-140 and 50-1000 U/L, respectively. The excellent performance of this novel biosensor demonstrated that this strategy has prodigious potential to be applied in practice detection of AChE.