Aquaporin-4 deficiency attenuates acute lesions but aggravates delayed lesions and microgliosis after cryoinjury to mouse brain

作     者：Wen-Zhen Shi Chun-Zhen Zhao Bing Zhao Xiao-Liang Zheng San-Hua Fang Yun-Bi Lu Wei-Ping Zhang Zhong Chen Er-Qing Wei 

作者机构：Department of Pharmacology and Institute of Neurobiology School of Medicine Zhejiang University Hangzhou 310058China 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2012年第28卷第1期

页      面：61-68页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 080202[工学-机械电子工程] 0802[工学-机械工程] 

基　　金：supported by the National Natural Science Foundation of China (81072618,30772561,30873053 and 30672449) the Natural Science Foundation of Zhejiang Province,China (Y2090069) the "Qian Jiang Ren Cai Research Plan" Zhejiang Province,China (2010R10055) Fundamental Research Funds for the Central Universities of China (2009QNA7008) 

主　　题：aquaporin-4 gene deficiency cryoinjury microglia astrocyte 

摘      要：Objective To determine whether aquaporin-4 （AQP4） regulates acute lesions, delayed lesions, and the associated microglial activation after cryoinjury to the brain. Methods Brain cryoinjury was applied to AQP4 knockout （KO） and wild-type mice. At 24 h and on days 7 and 14 after cryoinjury, lesion volume, neuronal loss, and densities of microglia and astrocytes were determined, and their changes were compared between AQP4 KO and wild-type mice. Results Lesion volume and neuronal loss in AQP4 KO mice were milder at 24 h following cryoinjury, but worsened on days 7 and 14, compared to those in wild-type mice. Besides, microglial density increased more, and astrocyte proliferation and glial scar formation were attenuated on days 7 and 14 in AQP4 KO mice. Conclusion AQP4 deficiency ameliorates acute lesions, but worsens delayed lesions, perhaps due to the microgliosis in the late phase.