The dependences of osteocyte network on bone compartment, age, and disease

作     者：Xiaohan Lai Christopher Price Shannon Modla William R Thompson Jeffrey Caplan Catherine B Kirn-Safran Liyun Wang 

作者机构：Department of Mechanical Engineering University of Delaware Biomedical Engineering Program University of Delaware DBI Bioimaging Center University of Delaware Department of Physical Therapy Indiana University Department of Biological Sciences University of Delaware 

出 版 物：《Bone Research》 (骨研究（英文版）)

年 卷 期：2015年第3卷第2期

页      面：72-82页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported partially by funds from the NIH (RO1AR054385  P30GM103333) 

主　　题：The dependences of osteocyte network on bone compartment age and disease bone 

摘      要：Osteocytes, the most abundant bone cells, form an interconnected network in the lacunar-canalicular pore system （LCS） buried within the mineralized matrix, which allows osteocytes to obtain nutrients from the blood supply, sense external mechanical signals, and communicate among themselves and with other cells on bone surfaces. In this study, we examined key features of the LCS network including the topological parameter and the detailed structure of individual connections and their variations in cortical and cancellous compa~ tments, at different ages, and in two disease conditions with altered mechanosensing （perlecan deficiency and diabetes）. LCS network showed both topological stability, in terms of conservation of connectivity among osteocyte lacunae （similar to the ＂nodes＂ in a computer network）, and considerable variability the pericellular annular fluid gap surrounding lacunae and canaliculi （similar to the ＂bandwidth＂ of individual links in a computer network）. Age, in the range of our study （15-32 weeks）, affected only the pericellular fluid annulus in cortical bone but not in cancellous bone. Diabetes impacted the spacing of the lacunae, while the perlecan deficiency had a profound influence on the pericellular fluid annulus. The LCS network features play important roles in osteocyte signaling and regulation of bone growth and adaptation.