Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences
Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences作者机构：State Key Laboratory for Infectious Disease Prevention and ControlNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases The First Affiliated HospitalCollege of MedicineZhejiang University Zhiyuan Medical Inspection Institute Co.Ltd. Sanmen People’s Hospital The First People’s Hospital of Xiaoshan District The First People’s Hospital of Cangnan Jinhua Municipal Central Hospital The First People’s Hospital of Pingyang The First Hospital of Jiaxing Zhoushan Hospital The First People’s Hospital of Wenling Rui’an People’s Hospital Maternal and Child Health Hospital of Zhoushan City Shangyu People’s Hospital People’s Hospital of Putuo DistrictZhoushan Hangzhou First People’s Hospital Qingtian People’s Hospital The First People’s Hospital of Yongkang The Children’s Hospital Zhejiang University School of Medicine Lishui People’s Hospital The Affiliated Hospital of Hangzhou Normal University Wuxi No.4 Hospital Affiliated to Suzhou University Jinhua Wenrong Hospital The First Affiliated Hospital of Wenzhou Medical College Yueqing People’s Hospital Tiantai People’s Hospital Lin’an people’s Hospital Rui’an City TCM Hospital Medical College of Hebei University of Engineering
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2015年第21卷第3期
页 面：944-952页
核心收录：
学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学]
基 金：Supported by Grants from the Science and Technology Program of Zhejiang Province China,No.2001C23140 National Technology RD Program in the 12th Five-Year Plan of China,No.2012BAI06B02 the Major Technology Project as part of"Prevention and Control of Major Infectious Diseases including AIDS and Viral Hepatitis",No.2013ZX10004216-002 the National Key Scientific Instrument and Equipment Development Project,No.2012YQ180117 the Medical and Health Science and Technology Plan Project of Zhejiang Province,No.2012KYB248 the Science and Technology Project of Zhejiang province,No.2011C23140
主 题：Centralized isolation Helicobacter pylori Influenc
摘 要：AIM:To evaluate the efficacy of centralized culture and possible influencing ***:From January 2010 to July 2012,66452 patients with suspected Helicobacter pylori(***) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal *** mucosal biopsies were taken from the antrum for *** biopsies were transported under natural environmental temperature to the central laboratory in Hangzhou city and divided into three groups based on their transport time:5,24 and 48 *** culture results were reported after 72 h and the positive culture rates were analyzed by a χ2 *** additional 5736 biopsies from ***-positive patients(5646 rapid urease test-positive and 90 14C-urease breath test-positive) were also cultured for quality control in the central laboratory ***:The positive culture rate was 31.66%(21036/66452) for the patient samples and 71.72%(4114/5736) for the ***-positive quality control *** the 5 h transport group,the positiveculture rate was 30.99%(3865/12471),and 32.84%(14960/45553) in the 24 h transport *** contrast,the positive culture rate declined significantly in the 48 h transport group(26.25%; P 0.001).During transportation,the average natural temperature increased from 4.67 to 29.14℃,while the positive culture rate declined from 36.67%(1462/3987) to 24.12%(1799/7459).When the temperature exceeded 24℃,the positive culture rate decreased significantly,especially in the 48 h transport group(23.17%).CONCLUSION:Transportation of specimens within 24 h and below 24℃ is reasonable and acceptable for centralized culture of multicenter *** samples.
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