Effects of sodium nitroprusside on callus browning of Ficus religiosa: an important medicinal plant

作     者：Mohsen Hesami Masoud Tohidfar Milad Alizadeh Mohammad Hosein Daneshvar Mohsen Hesami;Masoud Tohidfar;Milad Alizadeh;Mohammad Hosein Daneshvar

作者机构：Department of Horticultural ScienceFaculty of AgricultureUniversity of TehranKarajIran Department of Plant BiotechnologyFaculty of Life Science and BiotechnologyShahid Beheshti UniversityTehranIran Department of Horticultural ScienceFaculty of AgricultureKhuzestan Agricultural Sciences and Natural Resources UniversityMollasaniIran 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2020年第31卷第3期

页      面：789-796页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 10[医学] 

主　　题：Nitric oxide Oxidative enzymes Proline Reactive oxygen species 

摘      要：Tissue browning is a major problem in tissue culturing of woody plants, especially for Ficus religiosa which occurs by the accumulation and oxidation of phenolic compounds. This study aimed to determine the effect of different concentrations of sodium nitroprusside on the appearance of callus browning from leaf explants. The results indicate that callus browning was significantly reduced by supplementation of sodium nitroprusside to the MS medium and supplemented with 2.26μM of 2,4-dichlorophenoxyacetic acid and 0.22μM of 6-benzyl amino purine. The accumulation of hydrogen peroxide and phenolic compounds in the callus tissues decreased at the 50 μM concentration of sodium nitroprusside. Although catalase and peroxidase activities decreased at the 50μM concentration, the activity of superoxide dismutase and polyphenol oxidases, as well as proline content, increased exponentially. Sodium nitroprusside could be useful for the formation of non-embryogenic callus with high levels of metabolic activity for the production and isolation of secondary metabolites.