Bioinformatic identification of key genes and molecular pathways in the spermatogenic process of cryptorchidism
作者机构：Department of UrologyChildren’s Hospital of Chongqing Medical UniversityMinistry of Education Key Laboratory of Child Development and DisordersChongqing 400014China Chongqing Key Laboratory of Children Urogenital Development and Tissue EngineeringChina China International Science and Technology Cooperation Base of Child Development and Critical DisordersChina Chongqing Key Laboratory of PediatricsChina
出 版 物：《Genes & Diseases》 (基因与疾病（英文）)
年 卷 期：2019年第6卷第4期
页 面：431-440页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：The present study was financially supported by National Natural Science Foundation of China(Grant No.81771566) Entrepreneurship and Innovation Support Program for Returned Overseas Chinese Scholars,Chongqing(Grant No.cx2017015) Research and Innovation Project of Chongqing Graduate Students(Grant No.CYS17165)
主 题：Arachidonic acid pathway Bioinformatics Cryptorchidism Differentially expressed genes GPX8 mTOR RITOR Spermatogenic
摘 要：This study aims to determine key genes and pathways that could play important roles in the spermatogenic process of patients with *** gene expression profile data of GSE25518 was obtained from the Gene Expression Omnibus(GEO)*** data were analyzed using BRB-Array Tools to identify differentially expressed genes(DEGs)between high azoospermia risk(HAZR)patients and *** addition,other analytical methods were deployed,including hierarchical clustering analysis,class comparison between patients with HAZR and the normal control group,gene ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,and the construction of a proteineprotein interaction(PPI)*** total,1015 upregulated genes and 1650 downregulated genes were *** and KEGG analysis revealed enrichment in terms of changes in the endoplasmic reticulum cellular component and the endoplasmic reticulum protein synthetic process in the HAZR ***,the arachidonic acid pathway and mTOR pathway were also identified as important pathways,while RICTOR and GPX8 were indentified as key genes involved in the spermatogenic process of patients with *** present study,we found that changes in the synthesis of endoplasmic reticulum proteins,arachidonic acid and the mTOR pathway are important in the incidence and spermatogenic process of ***8 and RICTOR were also identified as key genes associated with ***,these data may provide novel clues with which to explore the precise etiology and mechanism underlying cryptorchidism and cryptorchidism-induced human infertility.
维普期刊数据库