Protein surface recognition of the novel tetra-carboxylphenyl calix[4]arene to cytochrome c

作     者：Wen Ting An Yong Jiao Xiao Hua Sun Chuan Dong Shao Min Shuang Ping Fang Xia Man Shing Wong 

作者机构：Research Center of Environmental Science and Engineering School of Chemistry and Chemical Engineering Shanxi University Taiyuan 030006 China Department of Chemistry Hong Kong Baptist University Kowloon Tong Hongkong S.A.R. China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2008年第19卷第11期

页      面：1341-1344页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (No.90813018) the Youth Foundation of Shanxi Province (No.2006021009) as well as the Youth Scientific and Technical Foundation of Shanxi University (Nos.2006007 and 2007112) 

主　　题：Tetra-carboxylphenyl calixarene Cytochromc c Protein surface recognition Fluorescence spectroscopy Molecular modeling 

摘      要：The interaction of the novel tetra-carboxylphenyl calix[4]arene （TCPC） with the bovine heart cytochrome c （Cc） was first investigated by fluorescence spectroscopy and molecular modeling methods. The formation of a stable 1：1 complex was monitored by fluorescence titration, and its binding constant is 1.916 ×10^7 L mol^-1. Molecular modeling reveals the recognition mechanism of TCPC to the Cc surface, that is, the electrostatic interaction drives TCPC to the Cc surface, and the van der Waals interaction orientates TCPC parallel to the cleft of Cc.