DAPT suppresses the proliferation of human glioma cell line SHG-44

作     者：Xin Liu Qiu-Ran Xu Wan-Fu Xie Mao-De Wang 

作者机构：Department of Neurosurgerythe First Affiliated Hospital of Medical College of Xi'an Jiaotong University Department of Hepatobiliary Surgerythe First Affiliated Hospital of Medical College of Xi'an Jiaotong University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2014年第7卷第7期

页      面：552-556页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Shaanxi Province Health Department Key Funds(sx201227273) 

主　　题：Human glioma cell SHG-44 cell line DAPT Notch signaling pathway 

摘      要：Objective:To explore the suppressing effect ofγ-secretase inhibitor DAPT on proliferation of human glioma cell line SHG-44 in vitro and its ***:The SHG-44 cell was treated by DAPT with different *** proliferation of cells was detected by MTT assay;cell cycle and TSC of CD133^+were determined by flow cytometry analysis technique;the key factor in Notch signaling pathway(Notch-1,Delta-1,Hes-1)was measured by reverse transcrip tase-polymerase chain reaction and western ***:DAPT inhibited the growth and proliferation of SHG-44 cells significantly(P0.05).And the inhibiting effect on SHG-44 cells produced by DAPT showed a dose-dependent *** increased the rate of cells in G_0/G_1 phase of SHG-44 cells,while it decreased the rate of cells in S *** of CD133^+was significantly reduced after DAPT treated SHC-44 *** expression of protein and mRNA of Notch-1,Delta-1 and Hes-1 were gradually downregulated with the increase of DAPT ***:DAPT can downregulate these key factor in Notch signaling pathway,reduce the TSC of CD133+and inhibit the proliferation of SHC-44 cells.