ABP41 is Involved in the Pollen Tube Development via Fragmenting Actin Filaments
ABP41 is Involved in the Pollen Tube Development via Fragmenting Actin Filaments作者机构：Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education Beijing Normal University Beijing 100875 People's Republic of China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2008年第1卷第6期
页 面：1048-1055页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
基 金：This work was supported by the National Basic Research Program of China (grant no. 2007CB108700 2006CB100100) and the National Natural Science Foundation of China (30630005 30470176)to H.R. No conflict of interest declared
主 题：ABP41 actin filament fragmentation pollen germination actin-binding protein.
摘 要：ABP41 is identified as a novel member of plant villin/gelsolin/fragmin superfamily proteins from lily pollen, which binds stoichiometrically to actin filaments and severs them in vitro. To further understand its in-vivo function and the potential molecular mechanisms, biochemical analysis, fluorescence microscopic observation and microinjection assays were performed. Different biochemical measurements showed that ABP41 maintained actin filaments in forms of short F-actin in vitro. Microinjection of ABP41 into pollen tubes could fragment the pre-existing actin filaments, inhibit the velocity of cytoplasmic streaming, and shorten the length of the clear zone of pollen tube. In addition, it was found that the endogenous ABP41 expressing level was dynamically corresponding to the short actin filament structure in pollen at different stages of pollen germination. Our results suggest that ABP41 is involved in the regulation of actin dynamics during the pollen germination process via maintenance of short dynamic actin filaments.
维普期刊数据库