Novel C-17 spirost protostane-type triterpenoids from Alisma plantago-aquatica with antiinflammatory activity in Caco-2 cells

作     者：Qinghao Jin Jianqing Zhang Jinjun Hou Min Lei Chen Liu Xia Wang Yong Huang Shuai Yao Bang Yeon Hwang Wanying Wu Dean Guo 

作者机构：Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China School of PharmacyShenyang Pharmaceutical UniversityShenyang 110016China University of Chinese Academy of SciencesBeijing 100049China College of PharmacyChungbuk National UniversityCheongju 2816South Korea 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2019年第9卷第4期

页      面：809-818页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 100602[医学-中西医结合临床] 10[医学] 

基　　金：The financial support from the National Standardization of Traditional Chinese Medicine Project(ZYBZH-C-SH-49,China) the National Natural Science Foundation of China(No.81530095) National Standardization Program for Chinese Medicine(ZYBZH-K-LN-01,China) Shanghai Committee of Science and Technology(16DZ0500800,China)are gratefully acknowledged 

主　　题：Alisma plantago-aquatica Linn. Protostane-type triterpenoids Caco-2 cells LPS-induced NO production 

摘      要：Twenty-one protostane-type triterpenoids with diverse structures, including nine new compounds(1 e9), were isolated from the of Alisma plantago-aquatica Linn. Structurally, alisolides A-F(1 e6), composed of an oxole group coupled to a five-membered ring, represent unusual C-17 spirost protostane-type triterpenoids. Alisolide H(8) is a novel triterpenoid with an unreported endoperoxide bridge. Alisolide I(9) represents the first example of 23,24-acetal triterpenoid. Their structures were elucidated based on spectroscopic analysis, wherein the absolute configurations of 4-6, 8 were further confirmed by the Mo2(OAc)4-induced ECD method. Furthermore, all isolates were evaluated for their inhibitory effects on LPS-induced NO production in Caco-2 cells, and all the compounds showed remarkable inhibitory activities, with IC50 values in the range of 0.76 e38.20 mmol/L.