Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions

作     者：Ana E.Gutierrez-Cabrera Wesley F.Zandberg Edgar Zenteno Mario H.Rodriguez Bertha Espinoza Carl Lowenberger 

作者机构：CONACyT-Centro de Investigacion Sobre Enfermedades InfecciosasInstituto Nacional de Salud PublicaCuernavacaMorelosMexico Department of ChemistrySimon Fraser UniversityBurnabyBCCanada Department of ChemistryThe University of British Columbia OkanaganKelownaBCCanada Facultad de MedicinaDepartamento de BioquimicaUniversidad Nacional Autdmoma de Mexico Mexico CityDFMexico Centro de Investigacion Sobre Enfermedades InfecciosasInstituto Nacional de Salud PublicaCuernavaca MorelosMexico Departamento de InmunologiaInstituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de Mexico Ciudad UniversitariaMexico CityDFMexico Department of Biological SciencesSimon Fraser UniversityBurnabyBCCanada 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2019年第26卷第5期

页      面：796-808页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：Simon Fraser University SFU 

主　　题：feeding conditions glycoproteins lectins perimicrovillar membrane triatomine vector-parasite interactions 

摘      要：Trypanosoma cruzi,the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage. Many studies suggest that the parasite binds to midgut-specific glycans. We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions. In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes. We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions. PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans. We propose that these molecules could be involved in the process of parasite-vector interactions.