MicroRNA expression in the hippocampal CA1 region under deep hypothermic circulatory arrest

作     者：Xiao-Hua Wang Dong-Xu Yao Xiu-Shu Luan Yu Wang Hai-Xia Liu Bei Liu Yang Liu Lei Zhao Xun-Ming Ji Tian-Long Wang 

作者机构：Department of AnesthesiologyXuanwu HospitalCapital Medical UniversityBeijingChina Institute of GeriatricsBeijingChina National Clinical Research Center for Geriatric DisordersBeijingChina Department of NeurosurgeryXuanwu HospitalCapital Medical UniversityBeijingChina Cerebrovascular Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2019年第14卷第11期

页      面：2003-2010页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 1010[医学-医学技术（可授医学、理学学位）] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81401084(to XHW) the Beijing Municipal Administration of Hospital Ascent Plan in China,No.DFL20150802(to TLW) the Beijing 215 High Level Healthcare Talent Plan Academic Leader in China,No.008-0027(to TLW) the Beijing Municipal Commission of Health and Family Planning in China,No.PXM2017_026283_000002(to TLW) the Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support in China,No.ZYLX201706(to TLW),303-01-005-0137-11(to TLW),65683.00(to TLW) 

主　　题：nerve regeneration cerebral protection deep hypothermic circulatory arrest gene ontology enrichment analysis microRNA hippocampus post-transcriptional expression microarray bioinformatics neural regeneration 

摘      要：Using deep hypothermic circulatory arrest, thoracic aorta diseases and complex heart diseases can be subjected to corrective procedures. However, mechanisms underlying brain protection during deep hypothermic circulatory arrest are unclear. After piglet models underwent 60 minutes of deep hypothermic circulatory arrest at 14°C, expression of microRNAs(miRNAs) was analyzed in the hippocampus by microarray. Subsequently, TargetScan 6.2, RNA22 v2.0, miRWalk 2.0, and miRanda were used to predict potential targets, and gene ontology enrichment analysis was carried out to identify functional pathways involved. Quantitative reverse transcription-polymerase chain reaction was conducted to verify miRNA changes. Deep hypothermic circulatory arrest altered the expression of 35 miRNAs. Twenty-two miRNAs were significantly downregulated and thirteen miRNAs were significantly upregulated in the hippocampus after deep hypothermic circulatory arrest. Six out of eight targets among the differentially expressed miRNAs were enriched for neuronal projection(cyclin dependent kinase, CDK16 and SLC1 A2), central nervous system development(FOXO3, TYRO3, and SLC1 A2), ion transmembrane transporter activity(ATP2 B2 and SLC1 A2), and interleukin-6 receptor binding(IL6 R)– these are the key functional pathways involved in cerebral protection during deep hypothermic circulatory arrest. Quantitative reverse transcription-polymerase chain reaction confirmed the results of microarray analysis. Our experimental results illustrate a new role for transcriptional regulation in deep hypothermic circulatory arrest, and provide significant insight for the development of miRNAs to treat brain injuries. All procedures were approved by the Animal Care Committee of Xuanwu Hospital, Capital Medical University, China on March 1, 2017(approval No. XW-INI-AD2017-0112).