Vitellaria paradoxa nutshells from seven sub-Saharan countries as potential herbal medicines for treating diabetes based on chemical compositions, HPLC fingerprints and bioactivity evaluation

作     者：LI Da XIAO Jian-Qi LIU Wen-Yuan ZHANG Chao-Feng AKIHISA Toshihiro ABE Masahiko MASTERS Eliot-T. ZHAI Wei-Wei FENG Feng ZHANG Jie 

作者机构：Jiangsu Food and Pharmaceutical Science CollegeHuai’an 223003China Department of Pharmaceutical AnalysisChina Pharmaceutical UniversityNanjing 210009China Research Institute for Science and TechnologyTokyo University of ScienceChiba 278-8510Japan World Agroforestry Centre(ICRAF)Nelson Marlbolough Institute of Technology(NMIT)Nelson 7010New Zealand School of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 211198China Key Laboratory of Biomedical Functional MaterialsChina Pharmaceutical UniversityNanjing 211198China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2019年第17卷第6期

页      面：446-460页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the General Program Foundation of Jiangsu Food and Pharmaceutical Science College(No.JSFP·2018005-Z) the Natural Science Foundation of Jiangsu Province(No.BK20170742) the Youth Science Fund Project of National Natural Science Foundation of China(No.81703383) 

主　　题：Vitellaria paradoxa HPLC fingerprints Antioxidant activity α-Glucosidase inhibitory activity Molecular docking 

摘      要：The aim of the study was to determine the feasibility of the Vitellaria paradoxa nutshell as a new medicinal resource for treating diabetes. A total of forty-one compounds were identified by HPLC-DAD-Q-TOF-MS and phytochemical methods in V. paradoxa nutshell methanol extract. Based on HPLC fingerprints, four characteristic constituents were quantified and the origin of twenty-eight V. paradoxa nutshells from seven sub-Saharan countries was compared, which were classified into three groups with chemometric method. Twenty-eight samples contained high total phenolic content, and exhibited moderate-higher antioxidant activity and strong α-glucosidase inhibitory activity. Furthermore, all fractions and isolated compounds were evaluated for their antioxidant and α-glucosidase inhibitory activities, and α-glucosidase inhibitory action mechanism of four characteristic constituents including protocatechuic acid, 3, 5, 7-trihydroxycoumarin,(2 R, 3 R)-(+)-taxifolin and quercetin was investigated via molecular docking method, which were all stabilized by hydrogen bonds with α-glucosidase. The study provided an effective approach to waste utilization of V. paradoxa nutshell, which would help to resolve waste environmental pollution and provide a basis for developing potential herbal resource for treating diabetes.