Effect of concentration and adsorption time on the formation of a large-scale origami pattern

作     者：Wen-Jing Liu Ping Zhang Tong Sun Lin Li Yu-Hui Wei Kai-Zhe Wang Lin Liu Bin Li 

作者机构：Division of Physical Biology and Bioimaging Centre Shanghai Synchrotron Radiation Facility CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China University of Chinese Academy of Sciences Beijing 100049 China Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai 201204 China School of Science Ningbo University Ningbo 315211 China 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2019年第30卷第7期

页      面：62-67页

核心收录：

学科分类：08[工学] 0827[工学-核科学与技术] 

基　　金：supported by the National Natural Science Foundation of China(No.31670871) the Open Large Infrastructure Research of Chinese Academy of Sciences the Chinese Academy of Sciences Knowledge Innovation Project(No.QYZDJ-SSW-SLH019) the LU JIAXI International team program supported by the K.C.Wong Education Foundation and CAS 

主　　题：DNA Origami Pattern Atomic force microscopy Close packing 

摘      要：The arrangement of DNA-based nanostructures into the desired large-scale periodic pattern with the highest possible accuracy and control is essential for the DNA application in functional biomaterials;however, formation of a DNA nanostructure pattern without utilizing the molecular interactions in nanotechnology field remains difficult. In this article, we use the optimal concentration and adsorption time of origami to induce DNA origami in the form of a large-scale 2D pattern on mica without changing the origami itself. DNA origami structures can form a pattern by close packing of symmetric and electrostatic interactions between ions, which was confirmed by the atomic force microscopy images. Furthermore, we identified favorable conditions for the concentration of enable pattern formation with DNA origami. This work provides an insight to understand the adsorption of DNA on mica and guides researches on regular DNA nanostructure pattern, which can serve as templates for pattern formation of proteins or other biomolecules.