Medium Term Survival of Bovine Caudal Epididymal Sperm under Conditions Based on Epididymal Fluid Characteristics

作     者：NI Jian-hong LIU Chang-bin ZHANG Bin Graham W KAY 

作者机构：Animal Husbandry Institute Xinjiang Academy of Agricultural Reclamation Science Shihezi 832000 China Agricultural and Life Sciences Division Lincoln University Christchurch 999030 New Zealand 

出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)

年 卷 期：2011年第3卷第1期

页      面：15-18页

学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

主　　题：Bovine Caudal epididymal sperm CEP-2 diluents 

摘      要：Fresh semen has a very limited life span after it leaves the gonad. The relative research shows that the epididymal plasma provides a special environment to spermatozoa for survival. In this study, the osmolarity and protein concentration of caput and caudal bull epididymis were analyzed. Bovine caudal epididymal sperm were incubated in different protein and sorbitol combinations of CEP-2 diluents at 4 ℃ for 120 h and sperm viability was assessed. Under this condition, sperm viability was greater than 60% after 5 d. The osmolarity of caput and caudal epididymis were （287.0 ± 13.7） and （310.8 ± 17.0 ） mOsm, respectively. The protein concentration of caput and caudal epididymis were （ 37.43 ± 12.55 ） and （50.58±11.08） mg/mg, respectively. This may have an application in the animal production industry.