Prussian blue nanosphere-embedded in situ hydrogel for photothermal therapy by peritumoral administration

作     者：Jijun Fu Bo Wu Minyan Wei Yugang Huang Yi Zhou Qiang Zhang Lingran Du 

作者机构：The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhou510700China Key Laboratory of Molecular Target&Clinical PharmacologySchool of Pharmaceutical SciencesGuangzhou Medical UniversityGuangzhou511436China Center of Pharmaceutical Research and DevelopmentSchool of Pharmaceutical SciencesGuangzhou Medical UniversityGuangzhou511436China 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2019年第9卷第3期

页      面：604-614页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 100602[医学-中西医结合临床] 

基　　金：supported by National Natural Science Foundation of China (81803463 and 51403043) the funding from Guangzhou Medical University (Grant No. B185004204, China) Guangzhou City Council (1201410511, China) and Guangdong Provincial Council (15KAL10, China) Munici-pal College Research Program of Guangzhou Education Bureau (1201610202, China) Guangdong Natural Science Foundation (2015A030310037, China) Guangdong Medical Science & Technology Research Foundation (A2017069, China) the Scientific Research Foundation of Guangzhou Education Bureau (1201610085 and 1201430509, China) Natural Science Foundation of Guangzhou Medical University (2014C09, China) 

主　　题：Prussian blue Nanospheres Hydrogel Thermosensitive Injectable In situ Photothermal 

摘      要：To establish an injectable hydrogel containing Prussian blue(PB) nanospheres for photothermal therapy against cancer, PB nanospheres were prepared by one-pot synthesis and the thermosensitive Pluronic F127 was used as the hydrogel matrix. The PB nanospheres and the hydrogel were characterized by shape, particle size, serum stability, photothermal performance upon repeated 808 nm laser irradiation, as well as the rheological features. The effect of the PB nanospheres and the hydrogel were evaluated qualitatively and quantitatively in 4T1 mouse breast cancer cells. The retention,photothermal efficacy, therapeutic effects and systemic toxicity of the hydrogel were assessed in a tumor-bearing mouse model. The PB nanospheres had a diameter of about 150 nm and exhibited satisfactory serum stability, photo-heat convert ability and repeated laser exposure stability. The hydrogel encapsulation did not negatively influence the above features of the photothermal agent. The nanospherecontaining hydrogel showed a phase transition at body temperature and, as a result, a long retention time in vivo. The photothermal agent-embedded hydrogel displayed promising photothermal therapeutic effects in the tumor-bearing mouse model with little-to-no systemic toxicity after peritumoral administration.