Effect of siRNA-mediated knockdown of eIF3c gene on survival of colon cancer cells

作     者：Ning SONG Yan WANG Xiao-dong GU Zong-you CHEN Liu-bin SHI 

作者机构：Department of General SurgeryHuashan HospitalFudan University 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2013年第14卷第6期

页      面：451-459页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Eukaryotic initiation factor subunit c(eIF3c) Colon cancer RKO cells Small interfering RNA(siRNA) 

摘      要：Eukaryotic initiation factor subunit c(eIF3c) has been identified as an oncogene that is over-expressed in tumor cells and,therefore,is a potential therapeutic target for gene-based cancer *** study was focused on investigating the effect of small interfering RNA(siRNA)-mediated eIF3c gene knockdown on colon cancer cell *** eIF3c gene was observed to be highly expressed in colon cancer cell *** expression levels of the gene in eIF3c siRNA infected and control siRNA infected cells were compared via real-time polymerase chain reaction(PCR) and western blotting *** proliferation levels were analyzed employing 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium bromide(MTT) and colony formation ***,the effects of eIF3c gene knockdown on the cell cycle and apoptosis were analyzed using flow *** results showed that suppression of eIF3c expression significantly(P0.001) reduced cell proliferation and colony formation of RKO colon cancer *** cell cycle was arrested by decreasing the number of cells entering S ***,apoptosis was induced as a result of eIF3c ***,eIF3c deletion effectively reduced the survival of colon cancer cells and could be used as a therapeutic tool for colon cancer therapy.