Integrative molecular characterization of Chinese prostate cancer specimens
作者机构:National Key Discipline of Human AnatomySchool of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China Guangdong Engineering Research Center for Translation of Medical 3D Printing ApplicationGuangzhou 510515China Department of UrologyNanfang HospitalSouthern Medical UniversityGuangzhou 510515China Department of OncologyAffiliated Cancer Hospital and Institute of Guangzhou Medical UniversityGuangzhou 510095China
出 版 物:《Asian Journal of Andrology》 (亚洲男性学杂志(英文版))
年 卷 期:2020年第22卷第2期
页 面:162-168页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:This work was mainly supported by the National Key R&D Program of China(No.2017YFC1103403) the National Natural Science Foundation of China(No.21773199,No.61427807 and No.81872092) the Sanming Project of Medicine in Shenzhen(No.SZSM201612019) the PhD Start-up Fund of Natural Science Foundation of Guangdong Province of China(No.2016A030310287)
主 题:androgen receptor molecular subtyping next generation sequencing prostate cancer quantitative real-time-polymerase chain reaction
摘 要:Prostate cancer(PCa)exhibits epidemiological and molecular *** extensive studies of its phenotypic and genetic properties in Western populations,its molecular basis is not clear in Chinese *** determine critical molecular characteristics and explore correlations between genomic markers and clinical parameters in Chinese populations,we applied an integrative genetic/transcriptomic assay that combines targeted next-generation sequencing and quantitative real-time PCR(qRT-PCR)on samples from 46 Chinese patients with ***(K)-specific methyltransferase 2D(KMT2D),zinc finger homeobox 3(ZFHX3),A-kinase anchoring protein 9(AKAP9),and GLI family zinc finger 1(GLI1)were frequently mutated in our ***,a clinicopathological analysis showed that RB transcriptional corepressor 1(RB1)deletion was common in patients with a high risk of disease ***,four genomic events,MYC proto-oncogene(MYC)amplification,RB1 deletion,APC regulator of WNT signaling pathway(APC)mutation or deletion,and cyclin-dependent kinase 12(CDK12)mutation,were correlated with poor disease-free *** addition,a close link between KMT2D expression and the androgen receptor(AR)signaling pathway was observed both in our cohort and in The Cancer Genome Atlas Prostate Adenocarcinoma(TCGA-PRAD)*** summary,our results demonstrate the feasibility and benefits of integrative molecular characterization of PCa samples in disease pathology research and personalized medicine.