Protection effect of Emodin pretreatment on intestinal I-RI damage of intestinal mucosa in ratsa

作     者：Shu-Jie Zhao Shi-Ji Wang Hong-Xiang Li Li-Hua Dong Huai-Jiang He Zhong-Min Liu Yu-Shan Wang 

作者机构：ICUFirst Hospital of Jilin University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2014年第7卷第9期

页      面：749-753页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by Special Project of Public Welfare Industry Affiliated to Ministry of Health Grant No:201202011 

主　　题：Emodin Ischemia-reperfusion injury Intestinal mucosa barrier Protection 

摘      要：Objective:To linvestigate the protective effect and mechanism of emodin pretreatment on intestinal mucosa of rats with intestinal ischemia-reperfusion ***:A total of 50SD rats were randomly divided into control group,model group,emodin groups of low,medium and high dose,with 10 in each ***-reperfusion injury(I-RI)mode was established by using noninvasive clamp on superior mesentericartery(SMA).Control group and model group were pretreated with 0.5%sodium carboxymethyl cellulose solution lavage 2 h before operation,emodin groups of low,medium and high dose were given emodin lavage with 20,40,60 mg/kg pretreatment,femoral venous blood before the lavage pretreatment(TO)and 1 h ischemia(Tl),and inferior vena venous blood after 1 h of reperfusion(T2)were extracted from each group of rats for detection of serun level of intestinal fatty acid binding protein(I-FABP),tumor necrosis factor(TNF-α),endotoxin,interleukin 6(IL-6),and die content of diamine oxidase(DAO);Mter model establishment,the rats were sacrificed,intestine homogenate was prepared by using blind intestinal tissue to detect intestinal tissue myeloperoxidase(MPO],malondialdehyde(MDA)and superoxide dismutase(SOD)*** upper small intestine tissue was retrieved,followed by fixation and conventional HE staining to observe intestinal tissue morphology under light ***:In emodin groups of low,medium and high dose at T1 and T2,I-FABP,TNF-α,***,-6 and DAO level were significandy lower than that of model group(P0.05);in emodin group of low,medium and high dose,MPO and MDA content in intestinal tissue homogenate was significantly lower than that in model group(P0.05),SOD level was significantly higher than that of model group(P0.05).Intestinal damage of emodin low,medium and high dose groups were significandy lighter than model ***:Emodin pretreatment has certain protective effect on intestinal mucosa in ischemia reperfusion injury.