人Era不同结构域在酵母双杂交系统中的表达及对报告基因激活作用的检测

作     者：吴元明 程轶梦 陈苏民 陈南春 纪宗玲 

作者机构：第四军医大学生物化学与分子生物学教研室西安710032 

出 版 物：《中国生物化学与分子生物学报》 (Chinese Journal of Biochemistry and Molecular Biology)

年 卷 期：2002年第18卷第2期

页      面：254-258页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 07[理学] 08[工学] 071007[理学-遗传学] 

基　　金：国家自然科学基金资助项目 (No .39870 380 396 70 0 0 6 )~~ 

主　　题：人Era 结构域 酵母双杂交系统 表达 报告基因 激活作用 检测 

摘      要：To construct the bait vector with different domains of human Era in yeast two-hybrid system, then assay whether its expression product can affect the growth of yeast cells and activate the reporter genes, the cDNA fragments encoding different domains of human Era was amplified by PCR, and subsequently they were cloned into pUC19. After being verified by sequencing, they were subcloned into the bait vector pGBKT7 of yeast two-hybrid system. Furthermore, these recombinant plasmids were transferred into AH109, and their expression products were assayed whether they could activate the reporter genes. The cDNA fragments encoding different domains of human Era were amplified successfully. The different domains of human Era were not toxic to AH109 and could not activate the reporter genes. Yeast two-hybrid GAL4 system could be used to fish human Era interacting protein.