Phenotypic characterization and genetic mapping of the dwarf mutant m34 in maize

作     者：LI Jie-ping Soomro Ayaz Ali XIAO Gui CHEN Fan-jun YUAN Li-xing GU Ri-liang 

作者机构：Lab of Plant-Soil Interaction Ministry of Agriculture/Department of Plant Nutrition College of Resources and Environmental Sciences China Agricultural University Center for Seed Science and Technology College of Agronomy and Biotechnology China Agricultural University 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2019年第18卷第5期

页      面：948-957页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by the National Key R&D Program of China(2016YFD0101803) the earmarked fund for China Agriculture Research System(CARS-02-10) the National Natural Science Foundation of China(31771891) the Chinese University Scientific Fund(2015ZH001) 

主　　题：dwarf plant height GA application ZmVP8 maize 

摘      要：Plant height is one of the most important agronomic traits associated with yield in *** this study,a gibberellins(GA)-insensitive dwarf mutant,m34,was screened from inbred line Ye478 by treatment with the chemical mutagen ethylmethanesulfonate(EMS).Compared to Ye478,m34 showed a dwarf phenotype with shorter internodes,and smaller leaf length and width,but with similar leaf ***,m34 exhibited smaller guard cells in internodes than Ye478,suggesting that smaller cells might contribute to its dwarf *** analysis indicated that the m34 dwarf phenotype was controlled by a recessive nuclear *** F2 population derived from a cross between m34 and B73 was used for mutational gene cloning and this gene was mapped to a chromosome region between umc2189 and umc1553 in chromosome 1 bin1.10,which harbored a previously identified dwarf gene Zm *** analysis showed a nucleotide substitution(G1606 to A1606)in the sixth exon of ZmVP8,which resulted in an amino acid change(E531 to K531)from Ye478 to *** amino acid change resulted in anα-helix changing to aβ-sheet in the secondary protein structure and the‘SPEC’domain changed to a‘BOT1NT’domain in the tertiary protein *** together,these results suggested that m34 is a novel allelic mutant originally derived from Ye478 that is useful for further ZmVP8 functional analysis in maize.