Agrobacterium-Mediated Transformation of Rice: Constraints and Possible Solutions

作     者：Sulaiman MOHAMMED Azman Abd SAMAD Zaidah RAHMAT 

作者机构：Department of Biotechnology and Medical Engineering Faculty of Biosciences and Medical Engineering Universiti Teknologi Malaysia Department of Biological Sciences Faculty of Science Gombe State University 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2019年第26卷第3期

页      面：133-146页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 0902[农学-园艺学] 

主　　题：rice Agrobacterium-mediated transformation tissue culture gene transfer T-DNA integration 

摘      要：Genetic transformation of rice(Oryza sativa L.) by introducing beneficial traits is now a central research instrument in plant physiology and a practical tool for plant improvement. Many approaches are verified for stable introduction of foreign genes into the plant genome. The review examined the different constraints that limit the success of rice genetic transformation via Agrobacterium-mediated approach and suggested possible solutions. Explant identification, gene transfer technique and construct to tailor the integration, transgene expression without collateral to genetic damage and transformant selection are among the technical challenges affecting the rice transformation. Despite the contests, Agrobacteriummediated transformation system has been a better option for producing transgenic rice varieties because of its exact T-DNA processing and simple integration of low copy-number transgene. This information is necessary for improving the transformation system for recalcitrant rice varieties.