Connexin 43 hemichannels protect bone loss during estrogen deficiency

作     者：Liang Ma Rui Hua Yi Tian Hongyun Cheng Roberto Jose Fajardo Joseph J. Pearson Teja Guda Daniel Brian Shropshire Sumin Gu Jean X. Jiang 

作者机构：Department of OrthopaedicsJinan Central Hospital Affiliated to Shandong UniversityJinanShandongChina Department of Biochemistry and Structural BiologyUT Health San AntonioSan AntonioTX 78229USA Department of OrthopedicsUT Health San AntonioSan AntonioTX 78229USA Department of Biomechanical EngineeringUniversity of Texas at San AntonioSan AntonioTX 78249USA 

出 版 物：《Bone Research》 (骨研究（英文版）)

年 卷 期：2019年第7卷第2期

页      面：183-194页

核心收录：

学科分类：10[医学] 

基　　金：supported by NIH grants,AR072020 and CA196214 Welch Foundation grant AQ-1507 to J.X.J China Scholarship Council funding to L.M.Micro-CT imaging was completed at RAYO,the Daniel Carlisle Center for Bone and Mineral Imaging at the University of Texas Health Science Center at San Antonio RAYO is supported by an equipment grant.R.J.F. was supported by NIH grant RR025687 

主　　题：hemichannels protect estrogen deficiency 

摘      要：Estrogen deficiency in postmenopausal women is a major cause of bone loss,resulting in osteopenia,osteoporosis,and a high risk for bone *** 43 (Cx43) hemichannels (HCs) in osteocytes play an important role in osteocyte viability,bone formation,and *** showed here that estrogen deficiency reduced Cx43 expression and HC *** determine if functional HCs protect osteocytes and bone loss during estrogen deficiency,we adopted an ovariectomy model in wild-type (WT) and two transgenic Cx43 mice:R76W (dominant-negative mutant inhibiting only gap junction channels) and Cx43 Δ130–136 (dominant-negative mutant compromising both gap junction channels and HCs).The bone mineral density (BMD),bone structure,and histomorphometric changes of cortical and trabecular bones after ovariectomy were *** results showed that the Δ130–136 transgenic cohort had greatly decreased vertebral trabecular bone mass compared to WT and R76W mice,associated with a significant increase in the number of apoptotic osteocyte and empty ***,osteoclast surfaces in trabecular and cortical bones were increased after ovariectomy in the R76W and WT mice,respectively,but not in Δ130–136 *** data demonstrate that impairment of Cx43 HCs in osteocytes accelerates vertebral trabecular bone loss and increase in osteocyte apoptosis,and further suggest that Cx43 HCs in osteocytes protect trabecular bone against catabolic effects due to estrogen deficiency.