A multiplatform metabolomic approach to characterize fecal signatures of negative postnatal events in chicks: a pilot study

作     者：Stéphane Beauclercq Antoine Lefèvre Frédéric Montigny Anne Collin Sophie Tesseraud Christine Leterrier Patrick Emond Laurence A.Guilloteau 

作者机构：BOAINRAUniversitéde Tours37380 NouzillyFrance Universitéde ToursPST Analyse des systèmes biologiquesToursFrance PRCINRACNRSUniversitéde ToursIFCE37380 NouzillyFrance UMR 1253iBrainUniversitéde ToursInsermToursFrance CHRU de ToursService de Médecine Nucléaire In VitroToursFrance. 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2019年第10卷第2期

页      面：424-434页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：supported financially by a “crédits incitatifs” grant from the department of Animal Physiology and Livestock Systems(PHASE)at INRA a grant from the Integrated Management of Animal Health metaprogram of INRA for the “GISA-WHELP” project(www.gisa.inra.fr/en) 

主　　题：Biomarker Chick Feces GC-MS LC-HRMS Negative postnatal event 

摘      要：Background: Negative experiences in early life can induce long-lasting effects on the welfare, health, and performance of farm animals. A delayed placement of chicks in rearing houses has negative effects on their performance, and results in fecal-specific odors detectable by rats. Based on this observation, the volatile organic compounds(VOCs) and metabolites from the feces of 12-day-old chickens were screened for early markers of response to negative events using gas-chromatography and liquid-chromatography coupled with mass spectrometry(GC-MS, LC-HRMS).Results: The low reproducibility of solid-phase micro-extraction of the VOCs followed by GC-MS was not suitable for marker discovery, in contrast to liquid extraction of metabolites from freeze-dried feces followed by GC-MS or LC-HRMS analysis. Therefore, the fecal metabolome from 12-day-old chicks having experienced a normal or delayed placement were recorded by GC-MS and LC-HRMS in two genotypes from two experiments. From both experiments, 25 and 35 metabolites, respectively explaining 81% and 45% of the difference between delayed and control chickens, were identified by orthogonal partial least-squares discriminant analysis from LC-HRMS and GC-MS ***: The sets of molecules identified will be useful to better understand the chicks’ response to negative events over time and will contribute to define stress or welfare biomarkers.