The Optimization of Ultrasonic-assisted Extraction of Polysaccharide from Sipunculus nudus by Response Surface Methodology and Study on Antioxidant Activity

作     者：Wen CHEN Xiangjun WANG Yujie WANG Sihui LIU Jiahui WEN Ruzheng CAI Weiqiang LIU Liuhuan LI 

作者机构：School of ocean science and technologyHainan Tropic Ocean University School of life science and ecologyHainan Tropic Ocean University 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2019年第8卷第1期

页      面：162-170页

学科分类：09[农学] 

基　　金：Supported by National Natural Science Foundation of Hainan Province(217157) 2017 Provincial College Students' Innovation and Entrepreneurship Training Program(201711100005) 2018 Provincial Key Discipline (Thalassochemistry) Construction Fund 

主　　题：Ultrasonic microwave Sipunculus nudus Polysaccharide Antioxidant activity 

摘      要：[Objectives]This study was conducted to optimize the extraction process of Sipunculus nudus polysaccharide and study the antioxidant activity of S. nudus polysaccharide. [Methods] S. nudus polysaccharide was extracted by ultrasonic microwave assisted method,during which univariate factors including solidliquid ratio,time,temperature,ultrasonic power,microwave power were investigated,to select optimal extracting conditions,which were also verified by an response surface optimization experiment( an four-factor three-level test); and the antioxidant activity of S. nudus polysaccharide was studied by determining its DPPH radical scavenging capacity. [Results]Through univariate factor investigation and response surface optimization test,it was found that the response surface results were consistent with univariate results. The optimized conditions were as follows: solid-liquid ratio at 1∶ 15,ultrasonic temperature at 60 ℃,ultrasonic power of 360 W and microwave power of 200 W,and the predicted polysaccharide extracting amount was 4. 35 mg/g. Under the optimized extracting conditions,the average extracting amount of S. nudus polysaccharide was 4. 38 mg/g,which is close to the theoretical predicted value( 4. 35 mg/g). And the results of this study also showed that different concentrations of polysaccharide solutions have different scavenging effects on DPPH free radical. At the polysaccharide concentration of0. 16 mg/ml,the maximum value of DPPH radical scavenging rate was 7. 22%,indicating that polysaccharide has certain antioxidant capacity. [Conclusions]This method is a new trial with satisfactory results.