Raman spectra of the GFP-like fluorescent proteins
Raman spectra of the GFP-like fluorescent proteins作者机构：National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences College of Life Science and Technology Huazhong Agricultural University College of Life Science Hubei University
出 版 物：《Biophysics Reports》 (生物物理学报(英文))
年 卷 期：2018年第4卷第5期
页 面：265-272页
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
基 金：supported by the Strategic Priority Research Program of Chinese Academy of Sciences (XDPB0305 CAS)
主 题：Chromophore Fluorescent protein Raman spectra
摘 要：The objective of the study was to elucidate optical characteristics of the chromophore structures of fluorescent proteins. Raman spectra of commonly used GFP-like fluorescent proteins（FPs） with diverse emission wavelengths（green, yellow, cyan and red）, including the enhanced homogenous FPs EGFP,EYFP, and ECFP（from jellyfish） as well as m Neptune（from sea anemone） were measured. High-quality Raman spectra were obtained and many marker bands for the chromophore of the FPs were identified via assignment of Raman spectra bands. We report the presence of a positive linear correlation between the Raman band shift of C;=C;and the excitation energy of FPs, demonstrated by plotting absorption maxima(cm;) against the position of the Raman band C;=C;in EGFP, ECFP, EYFP, the anionic chromophore and the neutral chromophore. This study revealed new Raman features in the chromophores of the observed FPs, and may contribute to a deeper understanding of the optical properties of FPs.
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