PROMOTIVE EFFECT OF Se ON THE GROWTH AND ANTIOXIDATION OF A BLUE-GREEN ALGA SPIRULINA MAXIMA

作     者：周志刚 刘志礼 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：1998年第16卷第4期

页      面：346-358页

核心收录：

学科分类：07[理学] 0713[理学-生态学] 

主　　题：ascorbate peroxidase glutathione peroxidase (GSH-POD) growth hydrogen peroxide (H₂O₂) hydroxyl radical (OH·) Se Spirulina maxima 

摘      要：Cultures of a blue-green alga Spirulina maxima （Setch. et Gard. ） Geitler with vari-ous concentrations of Se in Zarrouk’s medium showed that not higher than 40 mg/L Se could promote itsgrowth. The present experiments showed that S. maxima grown under normal conditions, has an oxidanstress defence system for hydrigen perovide （H2O2） removal, which is the Halliwell-Asada pathway.When 4 to 20 mg/L Se was added to the algal medium, this pathway was replaced by a so-called Se-stressed pathway containing GSH peroxidase （GSH-POD）. As a result of the occurrence of both higheractivity of GSH-POD and lower levels of hydroxy radical （OH）, the Se-stressed pathway scavengedH2O2 so effectively that the growth of S. maxima was promoted by 4 to 20 mg/L Se. While GSH-PODactivity of the alga disappeared at 40 mg/L Se, the recovery of ascothae peroxidase was observed. Thelower levels of ascorbic acid and GSH made the Hajliwell-Asada pathway for scavenging H2O2 less effec-tive, wlile the highest activity of catalase might be responsible in part for the H2O2 removal, causing thelevel of OH’ in S. maxima grown at 40 mg/L Se to be much higher than the OH. level in this algagrown at 4 to 20 mg/L Se, hut lower than that in the control. The OH’ level changes caused the growthof S. maxima cultured at 40 mg/L Se to increase slighil to close to that of the contrl.