Aberrant DNA methylation in cloned ovine embryos

作     者：LIU Lei HOU Jian LEI TingHua BAI JiaHua GUAN Hong AN XiaoRong 

作者机构：State Key Laboratories for Agrobiotechnology China Agricultural University Beijing 100094 China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2008年第53卷第3期

页      面：477-480页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：the National Natural Science Foundation of China(Grant No.30270956) High-Tech Research & Development Program of China(Grant No.2006AA02Z148) 

主　　题：绵羊晶胚 DNA甲基化 基因克隆 5-甲基胞核嘧啶 

摘      要：By using the approach of immunofluorescence staining with an antibody against 5-methylcytosine(5MeC),the present study detected the DNA methylation patterns of cloned ovine *** em-bryos derived from in vitro fertilization were also examined for reference *** results showed that:(1)during the pre-implantation development,cloned embryos displayed a similar demethylation profile to the fertilized embryos;that is,the methylation level decreased to the lowest at 8-cell stage,and then increased again at morulae ***,methylation level was obviously higher in cloned embryos than in stage-matched fertilized embryos,especially at 8-cell stage and afterwards;(2)at blastocyst stage,the methylation pattern in cloned embryos was different from that in fertilized *** cloned blastocyst,inner cell mass(ICM)exhibited a comparable level to trophectoderm cells(TE),while in in-vitro fertilized blastocyst the methylation level of ICM was lower than that of TE,which is not consistent with that reported by other *** results indicate that DNA methylation is abnormally reprogrammed in cloned embryos,implying that aberrant DNA methylation reprogramming may be one of the factors causing cloned embryos developmental failure.