Comparison of the Contributions of Tetrahydrofurfuryl Alcohol and PEG to a-Chymotrypsin Renaturation with High Performance Hydrophobic Interaction Chromatography

作     者：Ye Hua SHEN Hai Bo WANG Quan BAI Xin Du GENG 

作者机构：中国人民解放军85医院，上海200052 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2003年第14卷第3期

页      面：294-297页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：These projects No. 39880003 and 20175016 were supported by the National Natural Science Foundation of China 

主　　题：High-perforamnce hydrophobic interaction chromatography tetrahydrofurfuryl alcohol (THFA) polyethylene glycol (PEG) protein renaturation a-chymotrypsin. 

摘      要：The contributions of tetrahydrofurfuryl alcohol (THFA) and polyethylene glycol (PEG) to the renatured efficiency of a-chymotrypsin were investigated and compared with each other. The maximum increments of bioactivity recovery of a-Chy were found to be 25.1% for THFA, 10.4% for PEG, respectively. The experimental results indicated that the denaturant solution containing THFA contributed more to the renaturation of a-Chy in high performance hydrophobic interaction chromatography (HPHIC) than that containing PEG, when the concentration of THFA was 3.2%, the bioactivity recovery of a-Chy is the highest.