Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background

作     者：Ana Claudia Carstea Melinda K Pirity Andras Dinnyes 

作者机构：Genetic Reprogramming Group Agricultural Biotechnology CenterSzent Gyrgyi A u.4 H-2100 GdllHungary Biotalentum LtdAulich L. u 26H-2100 GdllHungary Molecular Animal Biotechnology Laboratory Szent Istvan UniversityPater K u 1H-2103GdllHungary 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2009年第1卷第1期

页      面：22-29页

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by Grants from EU FP6("MEDRAT"-LSHG-CT-2005-518240 "Artemis",LSHM-CT-2006-037862 "AGLAEA",LSHM-CT-2006-037554,"CLONET",MRTN-CT-2006-035468),EU FP7("PartnErS",PIAP-GA-2008-218205 "InduHeart",EUFP7-PEOPL E-IRG-2008-234390 "InduStem",PIAP-GA-2008-230675 "Plurisys",HEALTH-F4-2009-223485) NKFP_07_1-ES2HEART-HU,No.OM-00202-2007 NKTH/ANRTET Franco-Hungarian Bilateral Scientific and Technological Collaborative Project"Plurabit" 

主　　题：Chimeras Transgenic Embryonic stem cells Epigenetic changes Germline competence Induced pluripotent stem cells 

摘      要：In mice,gene targeting by homologous recombination continues to play an essential role in the understanding of functional *** strategy allows precise location of the site of transgene integration and is most commonly used to ablate gene expression(knock-out),or to introduce mutant or modified alleles at the locus of interest(knock-in).The efficacy of producing live,transgenic mice challenges our understanding of this complex process,and of the factors which influence germline competence of embryonic stem cell ***,evidence indicates that culture conditions and in vitro manipulation can affect the germline-competence of Embryonic Stem cell(ES cell) lines by accumulation of chromosome abnormalities and/or epigenetic alterations of the ES cell genome. The effectiveness of ES cell derivation is greatly strain-dependent and it may also influence the germline transmission *** technical improvements in the production of germline chimeras have been focused on means of generating ES cells lines with a higher germline *** are a number of options for generating chimeras from ES cells (ES chimera mice);however,each method has its advantages and *** developments in induced pluripotent stem(iPS)cell technology have opened new avenues for generation of animals from genetically modified somatic cells by means of chimera *** aim of this review is to give a brief account of how the factors mentioned above are influencing the germline transmission capacity and the developmental potential of mouse pluripotent stem cell *** most recent methods for generating specifically ES and iPS chimera mice,including the advantages and disadvantages of each method are also discussed.