检索结果-南通市图书馆

Comprehensive characterization of CRC with germline mutations reveals a distinct somatic mutational landscape and elevated cancer risk in the Chinese population

引用

Cancer Biology & Medicine 2022年第5期19卷 707-732页

作者： Jianfei Yao Yunhuan Zhen Jing Fan Yuan Gong Yumeng Ye Shaohua Guo Hongyi Liu Xiaoyun Li Guosheng Li Pan Yang Xiaohui Wang Danni Liu Tanxiao Huang Huiya Cao Peisu Suo Yuemin Li Jingbo Yu Lele Song Department of Radiotherapy the Eighth Medical Center of the Chinese PLA General HospitalBeijing 100091China HaploX Biotechnology Shenzhen 518057China Department of Colorectal Surgery Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004China International Business School Beijing Foreign Studies UniversityBeijing 100089China Department of Gastroenterology the Second Medical Center of the Chinese PLA General HospitalBeijing 100853China Department of Experimental Pathology Beijing Institute of Radiation MedicineBeijing 100850China Department of General Surgery the First Medical Center of the Chinese PLA General HospitalBeijing 100853China Department of Hepatobiliary Surgery Dalian Municipal Central HospitalDalian Medical UniversityDalian 116033China Comprehensive Liver Cancer Department the Fifth Medical Center of the Chinese PLA General HospitalBeijing 100039China

Objective:Hereditary colorectal cancer(CRC)accounts for approximately 5%–10%of all CRC cases.The full profile of CRC-related germline mutations and the corresponding somatic mutational profile have not been fully determined in the Chinese population.Methods:We performed the first population study investigating the germline mutation status in more than 1,000(n=1,923)Chinese patients with CRC and examined their relationship with the somatic mutational landscape.germline alterations were examined with a 58-gene next-generation sequencing panel,and somatic alterations were examined with a 605-gene panel.Results:A total of 92 pathogenic(P)mutations were identified in 85 patients,and 81 likely pathogenic(LP)germline mutations were identified in 62 patients,accounting for 7.6%(147/1,923)of all patients.MSH2 and APC was the most mutated gene in the Lynch syndrome and non-Lynch syndrome groups,respectively.Patients with P/LP mutations had a significantly higher ratio of microsatellite instability,highly deficient mismatch repair,family history of CRC,and lower age.The somatic mutational landscape revealed a significantly higher mutational frequency in the P group and a trend toward higher copy number variations in the non-P group.The Lynch syndrome group had a significantly higher mutational frequency and tumor mutational burden than the nonLynch syndrome group.Clustering analysis revealed that the Notch signaling pathway was uniquely clustered in the Lynch syndrome group,and the MAPK and cAMP signaling pathways were uniquely clustered in the non-Lynch syndrome group.Population risk analysis indicated that the overall odds ratio was 11.13(95%CI:8.289–15.44)for the P group and 20.68(95%CI:12.89–33.18)for the LP group.Conclusions:Distinct features were revealed in Chinese patients with CRC with germline mutations.The Notch signaling pathway was uniquely clustered in the Lynch syndrome group,and the MAPK and cAMP signaling pathways were uniquely clustered in the non-Lynch syndrome group.Patients with P/LP germline mutations

关键词： Colorectal cancer germline Lynch syndrome hereditary cancer next-generation sequencing Notch signaling pathway TMB MSI MMR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

DNA methylation dynamics during germline development

引用

Journal of Integrative Plant Biology 2022年第12期64卷 2240-2251页

作者： Shengbo He Xiaoqi Feng Guangdong Laboratory for Lingnan Modern Agriculture College of AgricultureSouth China Agricultural UniversityGuangzhou510642 China John Innes Centre Colney LaneNorwichNR47UH UK

DNA methylation plays essential homeostatic functions in eukaryotic genomes.In animals,DNA methylation is also developmentally regulated and,in turn,regulates development.In the past two decades,huge research effort has endorsed the understanding that DNA methylation plays a similar role in plant development,especially during sexual reproduction.The power of whole-genome sequencing and cell isolation techniques,as well as bioinformatics tools,have enabled recent studies to reveal dynamic changes in DNA methylation during germline development.Furthermore,the combination of these technological advances with genetics,developmental biology and cell biology tools has revealed functional methylation reprogramming events that control gene and transposon activities in flowering plant germlines.In this review,we discuss the major advances in our knowledge of DNA methylation dynamics during male and female germline development in flowering plants.

关键词： chromatin DNA methylation epigenetic reprogramming germline small interfering RNA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

germline promoter hypermethylation of tumor suppressor genes in gastric cancer

引用

World Journal of Gastroenterology 2012年第1期18卷 70-78页

作者： Pu-Yuan Wu Zheng Zhang Jing-Mei Wang Wen-Wen Guo Nong Xiao Qiong He Ya-Ping Wang Yi-Mei Fan Department of Medical Genetics Medical SchoolNanjing University Nanjing 210093 Jiangsu Province China Jiangsu Key Laboratory of Molecular MedicineNanjing 210093 Jiangsu Province China Department of Pathology Drum Tower Hos-pital Affiliated to Medical School Nanjing University Nanjing210093 Jiangsu Province China Departmem of Pathology Lujiang Hos-pital Lujiang Chaohu 231500 Anhui Province China

AIM: To explore germline hypermethylation of the tumor suppressor genes MLH1, CDH1 and P16INK4a in suspected cases of hereditary gastric cancer (GC). METHODS: A group of 140 Chinese GC patients in whom the primary cancer had developed before the age of 60 or who had a familial history of cancer were screened for germline hypermethylation of the MLH1, CDH1 and P16INK4a tumor suppressor genes. GenomicDNA was extracted from peripheral blood leukocytes and modified by sodium bisulfite. The treated DNA was then subjected to bisulfi te DNA sequencing for a specif ic region of the MLH1 promoter. The methylation status of CDH1 or P16INK4a was assayed using methylation-specif ic PCR. Clonal bisulf ite allelic sequencing in positive samples was performed to obtain a comprehensive analysis of the CpG island methylation status of these promoter regions. RESULTS: Methylation of the MLH1 gene promoter was detected in the peripheral blood DNA of only 1/140 (0.7%) of the GC patient group. However, this methylation pattern was mosaic rather than the allelic pattern which has previously been reported for MLH1 in hereditary non-polyposis colorectal cancer (HNPCC) patients. We found that 10% of the MLH1 alleles in the peripheral blood DNA of this patient were methylated, consistent with 20% of cells having one methylated allele. No germline promoter methylation of the CDH1 or P16INK4a genes was detected. CONCLUSION: Mosaic germline epimutation of the MLH1 gene is present in suspected hereditary GC patients in China but at a very low level. germline epimutation of the CDH1 or P16INK4a gene is not a frequent event.

关键词： Gastric cancer germline promoter methylation MLH1 CDH1 P16INK4a

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Utilization of central disk of blastoderm and germinal crescent region of interspecific germline chimera between chicken and quail

引用

Asian Journal of Andrology 2002年第2期4卷 83-86页

作者： TomokiSoh YukioKato 等 FacultyofAgriculture GraduateSchoolKyushuUniversityFukuoka812－8581Japan FacultyofAgriculture MeijiUniversityKawasaki214－8571Japan

OBJECTIVE:The production of interspecific germline chimeras between chicken and quail were attempted employing the dissociated cells derived from the blastodermal central disk (stage X) and the germinal crescent region of embryo (stage 7-8).;METHODS:The central disk (CD) of the area pellucida in chicken blastoderm (stage X) and the germinal crescent region (GCR) of embryo (stage 7-8) were dispersed and injected into the subgerminal cavity of quail blastoderm (stage X). Injected eggs were incubated for 7 days or to hatching. The donor chicken DNA was detected by the polymerase chain reaction.;RESULTS:In day-7 embryos, chicken DNA was detected in 5 gonads and 9 brains from 53 survived embryos received chicken CD cells, and 1 gonads and 6 brains from 27 survived embryos received chicken GCR. Chicken DNA was also detected from the semen of one adult male hatched from eggs received chicken GCR cells.;CONCLUSIONS:CD and GCR cells as the donors showed the possibility to produce the interspecific germline chimera, but further studies are needed to make necessary improvement.

关键词： Chicken Chimera germline Polymerase chain reaction Quail Semen

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prevalence and clinical significance of pathogenic germline BRCA1/2 mutations in Chinese non-small cell lung cancer patients

引用

Cancer Biology & Medicine 2019年第3期16卷 556-564页

作者： Xingsheng Hu Dongyong Yang Yalun Li Li Li Yan Wang Peng Chen Song Xu Xingxiang Pu Wei Zhu Pengbo Deng Junyi Ye Hanhan Zhang Analyn Lizaso Hao Liu Xinru Mao Hai Huang Qian Chu Chengping Hu Department of Medical Oncology Cancer HospitalChinese Academy of Medical SciencesBeijing100021China Department of Pulmonary Medicine Second Affiliated Hospital of Fujian Medical UniversityQuanzhou362100China Department of Respiratory Medicine West China HospitalSichuan UniversityChengdu610041China Department ofRespiratory Medicine Daping HospitalChongqing400042China Department of Respiratory Medicine Xinqiao HospitalArmy Medical UniversityChongqing400037China Department of Medical Oncology Tianjin Medical University CancerInstitute and HospitalNational Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin Tianjin's Clinical Research Center for Cancer Tianjin300060China Department of Thoracic Oncology TianjinMedical University General HospitalTianjin300052China Department of Medical Oncology Hunan Cancer HospitalChangsha410006China Department of Pathology Xiangya HospitalCentral South UniversityChangsha410008China Department of Respiratory Medicine Xiangya HospitalCentral South UniversityChangsha410008China Burning RockBiotech Guangzhou510300China Department of Oncology Tongji Hospital of Tongji Medical CollegeHuazhongUniversity of Science and TechnologyWuhan430022China

Objective: germline alterations in the breast cancer susceptibility genes type 1 and 2, BRCA1 and BRCA2, predispose individuals to hereditary cancers, including breast, ovarian, prostate, pancreatic, and stomach cancers.Accumulating evidence suggests inherited genetic susceptibility to lung cancer.The present study aimed to survey the prevalence of pathogenic germline BRCA mutations(gBRCAm) and explore the potential association between gBRCAm and disease onset in Chinese advanced non-small cell lung cancer(NSCLC) patients.Methods: A total of 6,220 NSCLC patients were screened using capture-based ultra-deep targeted sequencing to identify patients harboring germline BRCA1/2 mutations.Results: Out of the 6,220 patients screened, 1.03%(64/6,220) of the patients harbored the pathogenic gB RCAm, with BRCA2 mutations being the most predominant mutations(49/64, 76.5%).Patients who developed NSCLC before 50 years of age were more likely to carry gBRCAm(P = 0.036).Among the patients harboring classic lung cancer driver mutations, those with concurrent gBRCAm were significantly younger than those harboring the wild-type gBRCA(P = 0.029).By contrast, the age of patients with or without concurrent gBRCAm was comparable to those of patients without the driver mutations(P = 0.972).In addition, we identified EGFR-mutant patients with concurrent gBRCAm who showed comparable progression-free survival but significantly longer overall survival(P = 0.002) compared to EGFR-mutant patients with wild-type germline BRCA.Conclusions: Overall, our study is the largest survey of the prevalence of pathogenic gBRCAm in advanced Chinese NSCLC patients.Results suggested a lack of association between germline BRCA status and treatment outcome of EGFR-TKI.In addition,results showed a positive correlation between pathogenic gB RCAm and an early onset of NSCLC.

关键词： germline BRCA mutations non-small cell lung cancer prevalence BRCA1 BRCA2

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Human germline editing: Insights to future clinical treatment of diseases

引用

Protein & Cell 2019年第7期10卷 470-475页

作者： Yanni Li Xiang Jin Kang Jeremy Kah Sheng Pang Boon Seng Soh Yang Yu Yong Fan Key Laboratory for Major Obstetric Diseases of Guangdong Province Center of Reproductive Medicine The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 China Disease Modeling and Therapeutics Laboratory A*STAR Institute of Molecular and Cell Biology 61 Biopolis Drive Proteos Singapore 138673 Singapore Center of Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China

Last year, the first attempt to genetically modify human embryos in the United States was reported and sparked a huge debate (Ma et al., 2017). Although the first human germline modification was only performed two years ago, the study showed that rapid adva nces in tech no logy has allowed the rate of off-target effects and mosaicism to be reduced considerably (Liang et al., 2015). Recently, Vertex and CRISPR therapeutics collaborated and developed CTX001, the first CRISPR/Cas9-based therapy, targeting patients with P-thalassemia and have begun phase 1/2 clinical trials. With policies and technologies regarding genome editing both developing rapidly, explorations into the possibility of clinical gene editing for hundreds of hereditary diseases are starting to become achievable. Here, we address the progress of huma n embryo editi ng tech no logies so far and its promise and risks in advancing therapy for hereditary diseases.

关键词： Human germline editing Insights diseases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

germline genomes have a dominant-heritable contribution to cancer immune evasion and immunotherapy response

引用

Quantitative Biology 2020年第3期8卷 216-227页

作者： Xue Jiang Mohammad Asad Lin Li Zhanpeng Sun Jean-Sebastien Milanese Bo Liao Edwin Wang Shanghai Mental Health Center School of Biomedical EngineeringShanghai Jiao Tong UniversityShanghai 200030China Department of Biochemistry and Molecular Biology Cumming School of MedicineUniversity of CalgaryCalgaryT2N 4N1Canada Tianjin Normal University Tianjin 300074China Human Health Therapeutics Research Centre National Research Council CanadaMontrealH4P 2R2Canada School of Mathematics and Statistics Hainan Normal UniversityHaikou 570206China

Background:Immune evasion is a fundamental hallmark for cancer.At the early stages of tumor development,immune evasion strategies must be implemented by tumors to prevent attacks from the host immune systems.Blocking tumors5 immune evasion will re-activate the host immune systems to eliminate tumors.Immune-checkpoint therapy(ICT)which applies anti-PD-l/PD-Ll or anti-CTLA4 treatment has been a remarkable success in the past few years.However,〜70%of patients cannot gain any clinical benefits from ICT treatment due to the tumorimmunity system's complexity・In the past,germline pathogenic variants have been thought to have only minorheritable contributions to cancer.Results:Emerging evidence has shown that germline genomes play a dominant-heritable contribution to cancer via encoding the host immune system.The functional components of the immune system are encoded by the host genome,thus the germline genome might have a profound impact on cancer immune evasion and immunotherapy response.Indeed,recent studies showed that germline pathogenic variants can influence immune capacity in cancer patients at a population level by(i)shaping tumor somatic mutations,altering methylation patterns and antigen-presentation capacity or(ii)influencing NK cell's function to modulate lymphocyte infiltration in the tumor microenvironment.In addition,the HLA(types A,B or C)genotypes also shape the landscape of tumor somatic mutations.Conclusion:These results highlight the indispensable roles of germline genome in immunity and cancer development and suggest that germline genomics should be integrated into the research field of cancer biology and cancer immunotherapy.

关键词： germline genomics cancer immune evasion immunotherapy response

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Epidemiological characteristics and genetic alterations in adult diffuse glioma in East Asianpopulations

引用

Cancer Biology & Medicine 2022年第10期19卷 1440-1459页

作者： Zongchao Mo Junyi Xin Ruichao Chai Peter Y.M.Woo Danny T.M.Chan Jiguang Wang Division of Life Science and State Key Laboratory of Molecular Neuroscience The Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong SARChina HKUST Shenzhen-Hong Kong Collaborative Innovation Research Institute Shenzhen 518000China Department of Molecular Neuropathology Beijing Neurosurgical InstituteCapital Medical UniversityBeijing 100070China Department of Neurosurgery Kwong Wah HospitalHong Kong SARChina Hong Kong Neuro-Oncology Society Hong Kong SARChina Division of Neurosurgery Department of SurgeryPrince of Wales HospitalHong Kong SARChina Department of Chemical and Biological Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong SARChina Hong Kong Center for Neurodegenerative Diseases Hong Kong Science ParkHong Kong SARChina

Understanding the racial specificities of diseases—such as adult diffuse glioma,the most common primary malignant tumor of the central nervous system—is a critical step toward precision medicine.Here,we comprehensively review studies of gliomas in East Asian populations and other ancestry groups to clarify the racial differences in terms of epidemiology and genomic characteristics.Overall,we observed a lower glioma incidence in East Asians than in Whites;notably,patients with glioblastoma had significantly younger ages of onset and longer overall survival than the Whites.Multiple genome-wide association studies of various cohorts have revealed single nucleotide polymorphisms associated with overall and subtype-specific glioma susceptibility.Notably,only 3 risk loci—5p15.33,11q23.3,and 20q13.33—were shared between patients with East Asian and White ancestry,whereas other loci predominated only in particular populations.For instance,risk loci 12p11.23,15q15-21.1,and 19p13.12 were reported in East Asians,whereas risk loci 8q24.21,1p31.3,and 1q32.1 were reported in studies in White patients.Although the somatic mutational profiles of gliomas between East Asians and non-East Asians were broadly consistent,a lower incidence of EGFR amplification in glioblastoma and a higher incidence of 1p19q-IDH-TERT triple-negative low-grade glioma were observed in East Asian cohorts.By summarizing large-scale disease surveillance,germline,and somatic genomic studies,this review reveals the unique characteristics of adult diffuse glioma among East Asians,to guide clinical management and policy design focused on patients with East Asian ancestry.

关键词： Glioma East Asian epidemiology germline somatic

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

germline mutations in Thai patients with nonmucinous epithelial ovarian cancer

引用

World Journal of Clinical Oncology 2019年第11期10卷 358-368页

作者： Tarinee Manchana Prasit Phowthongkum Chinachote Teerapakpinyo Division of Gynecologic Oncology Department of Obstetrics and GynecologyFaculty of MedicineChulalongkorn University and King Chulalongkorn Memorial HospitalBangkok 10330Thailand Division of Medical Genetics Department of MedicineFaculty of MedicineChulalongkorn University and Medical Genetics CenterKing Chulalongkorn Memorial HospitalBangkok 10330Thailand Chulalongkorn GenePRO Center Research AffairsFaculty of MedicineChulalongkorn UniversityBangkok 10330Thailand

BACKGROUND Genetic testing is widely recommended for all epithelial ovarian cancer(EOC)patients.However,an increased probability of identifying germline mutations has been reported in selected patients with risk factors such as a family history or personal history of cancer and high-grade serous carcinoma(HGSC)subtype.HGSC has been reported to be the most common subtype of EOC worldwide(approximately 70%).However,this subtype is less prevalent in Thai patients(reported as only 20%).The difference in the distribution of various subtypes of EOC may reflect the incidence of germline mutations in Thai EOC patients.AIM To evaluate the frequencies of germline mutations in EOC patients and to compare the frequencies in those with and without clinical risk factors for hereditary ovarian cancer.METHODS This cross-sectional study included 112 nonmucinous EOC patients who underwent primary surgery at our tertiary care hospital.Clinical risk factors for hereditary ovarian cancer were defined as follows:Age below 40 years,a significant family history of cancer,synchronous ovarian and endometrial cancer,and HGSC.Comprehensive germline mutations were detected by nextgeneration sequencing.RESULTS Of a total of 112 patients,82(73.2%)patients had≥1 risk factor and 30(26.8%)patients had no risk factors.germline mutations were detected in 26 patients:20(17.8%)patients had BRCA1/2 mutations,but 6(5.4%)patients had mutations in other genes,including 1 in MLH1,1 in MSH2,1 in RAD51C,2 in ATM and 1 in CDH1.germline mutations were only detected in patients with risk factors(26 of 82,31.7%),not in patients without risk factors(Pgermline mutations(56.3%vs 10%for those with and without a history of cancer,respectively,40.8%vs 9.5%for those with and without HGSC).germline BRCA mutations were detected in 38.8%of patients with HGSC but in only 1.6%of those with non-HGSC.An age below 40 years,personal history

关键词： BRCA mutation Epithelial ovarian cancer germline mutation Thai

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Novel CDH1 germline mutations identified in Chinese gastric cancer patients

引用

World Journal of Gastroenterology 2013年第6期19卷 909-916页

作者： Qin-Hua Chen Wei Deng Xiao-Wei Li Xiu-Fang Liu Jing-Mei Wang Li-Feng Wang Nong Xiao Qiong He Ya-Ping Wang Yi-Mei Fan Department of Medical Genetics Medical SchoolNanjing University Jiangsu Key Laboratory of Molecular Medicine Department of Pathology Drum Tower Hospital Affiliated to Medical SchoolNanjing University Department of Oncology Drum Tower Hospital Affiliated to Medical SchoolNanjing University Lujiang Hospital

AIM:To give a comprehensive report of E-cadheringene (CDH1) variations in a population at a high risk for gastric cancer (GC).METHODS:The samples consisted of 178 men and 58 women with a mean age of 62.3 ± 9.4 years and an age range of 30-84 years.A total of 240 cancerfree controls were recruited (mean age of 61.8 ± 10.1 years,age range of 26-82 years).Samples were screened for CDH1 germline mutations by high-resolution melting analysis or directly sequencing.Luciferase reporter assay,RNA splicing assay and bioinformatic analysis were used to evaluate the effect of mutations.RESULTS:Four novel CDH1 sequence alterations were identified in GC patients including a G>T transition 49 bp before the start codon;a three-nucleotide deletion,c.44_46del TGC;one missense mutation,c.604G>A (V202I);and one variation in the intron,c.1320+7A>G.In addition,polymorphism frequencies were observed for CDH1-164delT,-161C>A,-73A>C,c.48+6C>T,c.48+62_48+63delinsCGTGCCCCAGCCC,c.894C>T (A298A),c.1224G>A (A408A),c.1888C>G (L630V),c.2076T>C (A692A),and c.2253C>T (N751N) which is similar to the data reported in http://www.***.nlm.***.gov/projects/SNP/.RNA splicing analysis suggested that the c.1320+7A>G and c.1224G>A variations did not affect exon splicing ability.Luciferase reporter assay demonstrated that the c.-49T variation might be helpful for E-cadherin transcription,though the increase in transcription activity is limited (only 33%).SIFT score and PolyPhen analysis both demonstrated that the L630V missense mutation probably damages protein function,while the V202I variant does not.CONCLUSION:This study reveals novel mutations in sporadic GC patients which had been poorly investigated for susceptibility genes.

关键词： Gastric cancer germline mutation CDH1 Luciferase reporter assay RNA splicing analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：