PowerPlex■ fusion 6C system: internal validation study

作     者：Ana Boavida Vanessa Bogas Lisa Sampaio Nair Gouveia Maria J.Porto Francisco Corte-Real Ana Boavida;Vanessa Bogas;Lisa Sampaio;Nair Gouveia;Maria J.Porto;Francisco Corte-Real

作者机构：Forensic Genetic ServiceCentre BranchNational Institute of Legal Medicine and Forensic SciencesCoimbraPortugal 

出 版 物：《Forensic Sciences Research》 (法庭科学研究（英文）)

年 卷 期：2018年第3卷第2期

页      面：130-137页

学科分类：0301[法学-法学] 03[法学] 

主　　题：Forensic science forensic genetics PowerPlex■Fusion 6C amplification short tandem repeat sequence(STRs) validation 

摘      要：This work is aimed at describing the proceedings and parameters used to validate PowerPlex■ Fusion 6C System,the polymerase chain reaction (PCR) amplification kit by Promega,for posterior implementation in the laboratorial routine of the Forensic Genetic *** PowerPlex■ Fusion 6C System allows multiplex PCR,through simultaneous amplification and posterior detection by fluorescence of 27 *** of the kit was made according to the laboratory s internal validation procedure based on validation guidelines from Scientific Working Group on DNA Analysis *** parameters were evaluated,such as specificity,analytical thresholds,sensitivity,precision,mixture studies,DNA control samples,a proficiency test and changes in the PCR-based procedures: final reaction volume and cycle number,changes in the reaction mixture for direct *** kit proved to be very robust and the results are in concordance with previous developmental validation by the *** some parameters,the results were better than expected.